1 00:00:01,900 --> 00:00:00,470 [Music] 2 00:00:22,720 --> 00:00:01,910 foreign 3 00:00:23,970 --> 00:00:22,730 [Music] 4 00:00:41,889 --> 00:00:23,980 [Applause] 5 00:00:44,930 --> 00:00:41,899 [Music] 6 00:00:48,049 --> 00:00:44,940 greetings friends fellow earthlings and 7 00:00:49,369 --> 00:00:48,059 fans of exploration welcome to ask an 8 00:00:51,590 --> 00:00:49,379 astrobiologist 9 00:00:53,990 --> 00:00:51,600 the show that celebrates the science and 10 00:00:56,090 --> 00:00:54,000 celebrates the scientists involved in 11 00:00:58,970 --> 00:00:56,100 our quest to understand the nature of 12 00:01:01,430 --> 00:00:58,980 life astrobiology where does life come 13 00:01:03,889 --> 00:01:01,440 from how does it change and evolve where 14 00:01:05,750 --> 00:01:03,899 is it located in our universe the 15 00:01:08,750 --> 00:01:05,760 questions we all want to know 16 00:01:11,450 --> 00:01:08,760 I am your host Dr Graham the cosmo 17 00:01:13,910 --> 00:01:11,460 biologist Lao and as always we're 18 00:01:16,130 --> 00:01:13,920 brought to you by segnet.org and the 19 00:01:18,109 --> 00:01:16,140 NASA astrobiology program 20 00:01:19,969 --> 00:01:18,119 as always we want to thank all of you as 21 00:01:22,429 --> 00:01:19,979 well for tuning in asking us questions 22 00:01:23,990 --> 00:01:22,439 during the live stream chat or if you're 23 00:01:25,310 --> 00:01:24,000 watching this later jumping over to the 24 00:01:28,010 --> 00:01:25,320 YouTube channel and adding your 25 00:01:29,270 --> 00:01:28,020 questions in I do my best several of us 26 00:01:31,190 --> 00:01:29,280 come through and look at those questions 27 00:01:32,870 --> 00:01:31,200 try to answer them when we can 28 00:01:34,130 --> 00:01:32,880 I'm so excited for this week now you 29 00:01:35,810 --> 00:01:34,140 might notice I'm in a slightly different 30 00:01:37,969 --> 00:01:35,820 environment I'm actually recording in 31 00:01:40,550 --> 00:01:37,979 Savannah Georgia uh for this month's 32 00:01:42,830 --> 00:01:40,560 episode but I'm so excited to have our 33 00:01:45,230 --> 00:01:42,840 guest on the show this month Dr Andrew 34 00:01:47,450 --> 00:01:45,240 sugar is joining us he's a scientist at 35 00:01:50,030 --> 00:01:47,460 the department of Plant Pathology at the 36 00:01:52,670 --> 00:01:50,040 University of Florida Dr sugar worked 37 00:01:54,410 --> 00:01:52,680 for 18 years the land a hydroponic 38 00:01:57,050 --> 00:01:54,420 research and education facility at 39 00:01:58,969 --> 00:01:57,060 Disney's Epcot Center as well as at 40 00:02:01,429 --> 00:01:58,979 NASA's Kennedy Space Center 41 00:02:03,770 --> 00:02:01,439 his research has gone in many different 42 00:02:05,330 --> 00:02:03,780 directions over the years it includes 43 00:02:07,310 --> 00:02:05,340 studying the effects of Martian 44 00:02:10,130 --> 00:02:07,320 conditions on the survival growth and 45 00:02:11,990 --> 00:02:10,140 adaptation of microorganisms as well as 46 00:02:14,750 --> 00:02:12,000 investigating things like methane on 47 00:02:16,910 --> 00:02:14,760 Mars as a potential biosignature and so 48 00:02:18,949 --> 00:02:16,920 I'm so excited to welcome Dr sugar and I 49 00:02:20,330 --> 00:02:18,959 hope you will as well Dr sugar thank you 50 00:02:21,710 --> 00:02:20,340 very much for joining us for asking 51 00:02:23,809 --> 00:02:21,720 astrobiologist 52 00:02:24,589 --> 00:02:23,819 oh hey it's my pleasure I'm glad to be 53 00:02:26,809 --> 00:02:24,599 here 54 00:02:27,949 --> 00:02:26,819 I'm like I said I'm so happy that you're 55 00:02:29,449 --> 00:02:27,959 joining us 56 00:02:32,150 --> 00:02:29,459 um looking through your bio you've done 57 00:02:33,470 --> 00:02:32,160 so many different things uh you earned 58 00:02:34,910 --> 00:02:33,480 your your bachelor's degree your 59 00:02:36,290 --> 00:02:34,920 master's degree at the University of 60 00:02:37,910 --> 00:02:36,300 Arizona 61 00:02:39,650 --> 00:02:37,920 um then you worked during your PhD you 62 00:02:41,750 --> 00:02:39,660 earned that in 1991 you know the 63 00:02:44,270 --> 00:02:41,760 University of Florida working in this 64 00:02:47,270 --> 00:02:44,280 realm of microbiology microbial ecology 65 00:02:49,190 --> 00:02:47,280 and then Plant Pathology I'm wondering 66 00:02:52,009 --> 00:02:49,200 for our audience could you explain what 67 00:02:55,070 --> 00:02:52,019 really drove you towards these Realms of 68 00:02:57,949 --> 00:02:55,080 like microbiology and Plant Pathology 69 00:03:01,430 --> 00:02:57,959 well uh the biggest reason I got into 70 00:03:03,550 --> 00:03:01,440 Plant Pathology is I immediately had the 71 00:03:06,770 --> 00:03:03,560 sense that it was like being a detective 72 00:03:09,589 --> 00:03:06,780 because you have a series of problems 73 00:03:12,229 --> 00:03:09,599 with a plant or crop there are symptoms 74 00:03:15,050 --> 00:03:12,239 or signs you have to go into it and kind 75 00:03:17,809 --> 00:03:15,060 of use all of your knowledge from every 76 00:03:19,250 --> 00:03:17,819 course you've taken from every uh you 77 00:03:21,710 --> 00:03:19,260 know bit of experience that you've been 78 00:03:23,390 --> 00:03:21,720 able to Garner and then figure out what 79 00:03:26,330 --> 00:03:23,400 the problem is and then it's another 80 00:03:28,009 --> 00:03:26,340 that's first level of detective work and 81 00:03:30,170 --> 00:03:28,019 then the next level is to figure out how 82 00:03:31,729 --> 00:03:30,180 to control that disease so I just 83 00:03:34,850 --> 00:03:31,739 thought it'd be it was a very 84 00:03:37,250 --> 00:03:34,860 challenging Innovative discipline and I 85 00:03:38,570 --> 00:03:37,260 I've been very pleased with a career at 86 00:03:40,550 --> 00:03:38,580 Plant Pathology 87 00:03:42,410 --> 00:03:40,560 it's wonderful and of course you're not 88 00:03:44,149 --> 00:03:42,420 University of Florida but even while 89 00:03:46,670 --> 00:03:44,159 working on your PhD you were working at 90 00:03:48,410 --> 00:03:46,680 the land at Epcot Center kind of in this 91 00:03:49,970 --> 00:03:48,420 realm of hydroponic research and 92 00:03:52,130 --> 00:03:49,980 education 93 00:03:53,570 --> 00:03:52,140 um looking in these Management Systems 94 00:03:55,490 --> 00:03:53,580 um I'm wondering what you know how did 95 00:03:57,170 --> 00:03:55,500 you kind of evolve your career into that 96 00:03:59,449 --> 00:03:57,180 realm of like looking in closed loop 97 00:04:00,589 --> 00:03:59,459 systems and hydroponic systems and kind 98 00:04:02,630 --> 00:04:00,599 of using these as a way to understand 99 00:04:04,610 --> 00:04:02,640 Plant Pathology 100 00:04:07,910 --> 00:04:04,620 well that's a very interesting question 101 00:04:11,210 --> 00:04:07,920 because when I uh before I applied for 102 00:04:14,449 --> 00:04:11,220 the position at Epcot Center at the land 103 00:04:16,969 --> 00:04:14,459 Pavilion that I eventually got uh I was 104 00:04:19,729 --> 00:04:16,979 very interested in going into nature 105 00:04:21,469 --> 00:04:19,739 cinematography but my department chair 106 00:04:23,030 --> 00:04:21,479 and Plant Pathology at the University of 107 00:04:25,790 --> 00:04:23,040 Arizona tracked me down on the hall 108 00:04:28,249 --> 00:04:25,800 carrying a sheet of paper and he said 109 00:04:31,070 --> 00:04:28,259 this job's for you and it was the 110 00:04:35,170 --> 00:04:31,080 announcement of working in a hydroponic 111 00:04:38,090 --> 00:04:35,180 complex hydroponic facility up to 40 50 112 00:04:41,570 --> 00:04:38,100 crops agronomic and Horticultural crops 113 00:04:43,550 --> 00:04:41,580 and I immediately right when that that 114 00:04:46,189 --> 00:04:43,560 department chair showed me that piece of 115 00:04:49,189 --> 00:04:46,199 paper made the connection that that is 116 00:04:51,770 --> 00:04:49,199 the is sort of like a ground test bed 117 00:04:55,430 --> 00:04:51,780 for trying to learn how to grow plants 118 00:04:57,530 --> 00:04:55,440 in outer space so that was the reason I 119 00:05:00,290 --> 00:04:57,540 applied was that NASA was here in 120 00:05:03,230 --> 00:05:00,300 Florida Kennedy Space Center was here in 121 00:05:05,749 --> 00:05:03,240 Florida Epcot was here in Florida and 122 00:05:08,210 --> 00:05:05,759 this facility growing plants 123 00:05:10,310 --> 00:05:08,220 hydroponically was like a ground test 124 00:05:12,590 --> 00:05:10,320 bed for doing it in space so I said I 125 00:05:14,030 --> 00:05:12,600 have to try and I was lucky enough to 126 00:05:16,370 --> 00:05:14,040 get it and it's been it was a real 127 00:05:18,890 --> 00:05:16,380 rewarding experience working for Walt 128 00:05:20,689 --> 00:05:18,900 Disney World well that's really cool I 129 00:05:22,129 --> 00:05:20,699 do have to ask you know since my I 130 00:05:24,770 --> 00:05:22,139 really love this realm not just of 131 00:05:26,450 --> 00:05:24,780 astrobotany but also the future of 132 00:05:27,950 --> 00:05:26,460 Agriculture 133 00:05:29,330 --> 00:05:27,960 um what your view is kind of on the 134 00:05:31,969 --> 00:05:29,340 future of like things like vertical 135 00:05:33,590 --> 00:05:31,979 farms and indoor greenhouses do you 136 00:05:35,990 --> 00:05:33,600 think we kind of need to go that 137 00:05:38,029 --> 00:05:36,000 direction for not just being sustainable 138 00:05:40,909 --> 00:05:38,039 for space exploration but for building a 139 00:05:43,010 --> 00:05:40,919 future for humans as well here on Earth 140 00:05:47,210 --> 00:05:43,020 well that's a good question too because 141 00:05:50,870 --> 00:05:47,220 uh vertical farming has a real important 142 00:05:53,510 --> 00:05:50,880 role to play in urban areas where you're 143 00:05:56,749 --> 00:05:53,520 growing High cash value crops 144 00:06:00,170 --> 00:05:56,759 strawberries lettuce dwarf Tomatoes 145 00:06:02,330 --> 00:06:00,180 things of that nature such that you have 146 00:06:05,529 --> 00:06:02,340 a very short distance from production 147 00:06:07,730 --> 00:06:05,539 facility to sales facility 148 00:06:09,770 --> 00:06:07,740 but you have to it's generally 149 00:06:11,450 --> 00:06:09,780 economical only if it's a high cash 150 00:06:14,870 --> 00:06:11,460 value crop like the ones I just 151 00:06:17,590 --> 00:06:14,880 mentioned for bulk growth of plants like 152 00:06:20,870 --> 00:06:17,600 wheat potatoes some of the Staples 153 00:06:22,909 --> 00:06:20,880 that's probably going to remain for the 154 00:06:26,090 --> 00:06:22,919 foreseeable future as a field 155 00:06:28,909 --> 00:06:26,100 agriculture uh technology so we really 156 00:06:31,249 --> 00:06:28,919 need both we need solid field 157 00:06:33,890 --> 00:06:31,259 agriculture with good control of soils 158 00:06:35,650 --> 00:06:33,900 and pests and and proper nutritional 159 00:06:38,510 --> 00:06:35,660 management of the crops 160 00:06:41,689 --> 00:06:38,520 ecologically sound growing as well as 161 00:06:43,969 --> 00:06:41,699 vertical farmings for a very targeted uh 162 00:06:47,529 --> 00:06:43,979 communities but the the link here is 163 00:06:50,689 --> 00:06:47,539 vertical farming is a very good 164 00:06:53,570 --> 00:06:50,699 proxy for learning how to grow plants in 165 00:06:55,969 --> 00:06:53,580 really small spaces under really tight 166 00:06:57,830 --> 00:06:55,979 light and temperature controls that 167 00:07:01,490 --> 00:06:57,840 would extrapolate to growing on the 168 00:07:02,990 --> 00:07:01,500 surface of Mars or in a habitat on on 169 00:07:04,790 --> 00:07:03,000 the moon 170 00:07:06,170 --> 00:07:04,800 oh that's wonderful yeah there's there's 171 00:07:08,570 --> 00:07:06,180 so much we need to know for better 172 00:07:10,550 --> 00:07:08,580 growing food in our spacecraft you know 173 00:07:13,010 --> 00:07:10,560 we've now grown plants on the ISS that 174 00:07:15,290 --> 00:07:13,020 astronauts have eaten you know homegrown 175 00:07:17,629 --> 00:07:15,300 food on the ISS but it feels like that 176 00:07:18,830 --> 00:07:17,639 realm is still kind of developing 177 00:07:20,990 --> 00:07:18,840 um what do you think are some of the key 178 00:07:23,270 --> 00:07:21,000 things we need to know right now for the 179 00:07:25,129 --> 00:07:23,280 next maybe decade or two and when it 180 00:07:27,469 --> 00:07:25,139 comes to growing plants on a space 181 00:07:30,710 --> 00:07:27,479 station moon or on Mars 182 00:07:33,529 --> 00:07:30,720 right well uh the number one thing is 183 00:07:35,029 --> 00:07:33,539 that uh I I think a lot of your viewers 184 00:07:37,909 --> 00:07:35,039 are probably seeing a lot of very 185 00:07:40,430 --> 00:07:37,919 complex illustrations artist renderings 186 00:07:43,730 --> 00:07:40,440 of a moon base with a very complex 187 00:07:46,610 --> 00:07:43,740 agricultural system inside or Mars base 188 00:07:50,089 --> 00:07:46,620 with something similar but that's the 189 00:07:53,689 --> 00:07:50,099 target that's the goal 10 20 years down 190 00:07:55,850 --> 00:07:53,699 the road right now though we're really 191 00:07:58,189 --> 00:07:55,860 focused we being the community of 192 00:08:00,650 --> 00:07:58,199 astrobiology and plant biology in space 193 00:08:03,230 --> 00:08:00,660 we're really focused on trying to 194 00:08:05,270 --> 00:08:03,240 optimize plant growth in the 195 00:08:08,029 --> 00:08:05,280 microgravity environment on Space 196 00:08:09,529 --> 00:08:08,039 Station uh the number of plant growing 197 00:08:12,770 --> 00:08:09,539 systems have been developed they've 198 00:08:15,589 --> 00:08:12,780 flown and to me as a plant pathologist 199 00:08:18,469 --> 00:08:15,599 one of the most intriguing issues that 200 00:08:20,990 --> 00:08:18,479 is does not have a lot of research is 201 00:08:23,210 --> 00:08:21,000 how do the microorganisms present in 202 00:08:25,610 --> 00:08:23,220 let's say the space station or 203 00:08:28,369 --> 00:08:25,620 eventually those same microorganisms 204 00:08:30,770 --> 00:08:28,379 might be in a a biogenerative life 205 00:08:32,630 --> 00:08:30,780 support system on the moon or Mars how 206 00:08:35,630 --> 00:08:32,640 do those microbes interact with the 207 00:08:38,570 --> 00:08:35,640 plants and is the space environment so 208 00:08:41,089 --> 00:08:38,580 stressful to the plants that host 209 00:08:43,969 --> 00:08:41,099 resistance that we normally expect from 210 00:08:46,430 --> 00:08:43,979 a plant would that break down because of 211 00:08:48,470 --> 00:08:46,440 the stresses in microgravity and that's 212 00:08:51,110 --> 00:08:48,480 an area that I'm actively doing research 213 00:08:53,810 --> 00:08:51,120 right now and it's it's quite enjoyable 214 00:08:56,210 --> 00:08:53,820 and and quite challenging that's 215 00:08:58,310 --> 00:08:56,220 fantastic and I I do want to talk then a 216 00:08:59,509 --> 00:08:58,320 bit about how things will survive on 217 00:09:00,949 --> 00:08:59,519 Mars 218 00:09:02,870 --> 00:09:00,959 um you know Earth life we we are 219 00:09:04,310 --> 00:09:02,880 protected here on our planet by this 220 00:09:06,710 --> 00:09:04,320 beautiful magnetic field that's 221 00:09:08,810 --> 00:09:06,720 generated by the Dynamo of the outer 222 00:09:10,790 --> 00:09:08,820 core of our world you know and I think 223 00:09:12,170 --> 00:09:10,800 some people forget sometimes that you 224 00:09:14,509 --> 00:09:12,180 know astronauts and other terrestrial 225 00:09:16,430 --> 00:09:14,519 organisms going to Mars they're going to 226 00:09:18,350 --> 00:09:16,440 face a different radiation environment 227 00:09:20,750 --> 00:09:18,360 at the surface of Mars as well as things 228 00:09:22,070 --> 00:09:20,760 like the very low pressure the much 229 00:09:24,590 --> 00:09:22,080 lower temperature it's an average of 230 00:09:26,210 --> 00:09:24,600 negative 90 Fahrenheit at the surface of 231 00:09:28,310 --> 00:09:26,220 Mars even though that does drastically 232 00:09:30,050 --> 00:09:28,320 change around the world 233 00:09:31,910 --> 00:09:30,060 um but first for our audience who are 234 00:09:33,530 --> 00:09:31,920 watching live we have a quick pull to 235 00:09:35,389 --> 00:09:33,540 drop in the YouTube chat for those of 236 00:09:37,130 --> 00:09:35,399 you who are watching right now 237 00:09:39,170 --> 00:09:37,140 um we have a question about radiation 238 00:09:41,630 --> 00:09:39,180 and how it affects not just humans but 239 00:09:44,750 --> 00:09:41,640 some of the extremophiles of our world 240 00:09:46,910 --> 00:09:44,760 as well so we humans we get sick with 241 00:09:48,470 --> 00:09:46,920 radiation when we get a dose that's 242 00:09:50,690 --> 00:09:48,480 actually pretty close to the dosage we 243 00:09:55,009 --> 00:09:50,700 would get on the surface of Mars around 244 00:09:56,810 --> 00:09:55,019 0.3 Grays or so a gray is one joule per 245 00:09:59,329 --> 00:09:56,820 kilogram that's the absorbed dose of 246 00:10:00,829 --> 00:09:59,339 radiation and so our question for you in 247 00:10:02,870 --> 00:10:00,839 the YouTube chat and we'll come back to 248 00:10:05,050 --> 00:10:02,880 this in a little bit is how much 249 00:10:08,269 --> 00:10:05,060 radiation do you think the extremophile 250 00:10:09,590 --> 00:10:08,279 dinococcus radio durans can handle and 251 00:10:11,690 --> 00:10:09,600 you'll see the answers popping up right 252 00:10:13,009 --> 00:10:11,700 now in your chat uh Andrew and I can't 253 00:10:14,449 --> 00:10:13,019 see those answers but we'll come back to 254 00:10:15,949 --> 00:10:14,459 that in a little bit 255 00:10:17,090 --> 00:10:15,959 um okay Andrew from you I just want to 256 00:10:18,590 --> 00:10:17,100 hear you know what your what your 257 00:10:20,870 --> 00:10:18,600 research has been kind of looking in 258 00:10:23,030 --> 00:10:20,880 this realm of survival and growth and 259 00:10:25,430 --> 00:10:23,040 the adaptation organisms have had to use 260 00:10:29,150 --> 00:10:25,440 to survive in Martian conditions 261 00:10:30,949 --> 00:10:29,160 all right so I sent a slide uh with the 262 00:10:35,030 --> 00:10:30,959 Mars chamber on it if you could just pop 263 00:10:36,949 --> 00:10:35,040 that up briefly uh I I've designed and 264 00:10:38,930 --> 00:10:36,959 built a mar simulation chamber here in 265 00:10:42,590 --> 00:10:38,940 my lab I've been working with it almost 266 00:10:44,810 --> 00:10:42,600 20 years and it is able to to recreate 267 00:10:47,269 --> 00:10:44,820 five conditions on the surface of the 268 00:10:49,370 --> 00:10:47,279 current Demars low pressure which is 269 00:10:52,250 --> 00:10:49,380 around seven millibars I'm in Florida 270 00:10:54,530 --> 00:10:52,260 near the sea level uh near sea level so 271 00:10:57,949 --> 00:10:54,540 I'm breathing right now 1000 and 15 272 00:11:01,130 --> 00:10:57,959 millibars but the surface of Mars 273 00:11:03,949 --> 00:11:01,140 is actually seven to ten millibar so 274 00:11:06,470 --> 00:11:03,959 it's less than one percent of sea level 275 00:11:09,889 --> 00:11:06,480 pressure on Earth uh so that's the first 276 00:11:11,509 --> 00:11:09,899 Factor second is UV irradiation very 277 00:11:13,550 --> 00:11:11,519 strong ultraviolet irradiation 278 00:11:16,790 --> 00:11:13,560 environment on Mars because there's 279 00:11:18,530 --> 00:11:16,800 literally no ozone layer uh in the 280 00:11:20,810 --> 00:11:18,540 Martian atmosphere which allows a lot of 281 00:11:24,350 --> 00:11:20,820 the biocidal UV to hit the surface 282 00:11:26,630 --> 00:11:24,360 number three is temperature uh gas 283 00:11:28,370 --> 00:11:26,640 compositions number four and then the 284 00:11:31,130 --> 00:11:28,380 Mars chamber also has a way of 285 00:11:33,769 --> 00:11:31,140 simulating either dust free conditions 286 00:11:35,990 --> 00:11:33,779 on mars or Global dust storm conditions 287 00:11:37,850 --> 00:11:36,000 and everything in between so what I've 288 00:11:41,030 --> 00:11:37,860 done if you can then switch to me for a 289 00:11:43,210 --> 00:11:41,040 close-up I I have these little microbial 290 00:11:46,730 --> 00:11:43,220 sample holders this is just one example 291 00:11:49,910 --> 00:11:46,740 and this a little piece of aluminum in 292 00:11:52,730 --> 00:11:49,920 here is what's called a coupon and upon 293 00:11:54,470 --> 00:11:52,740 each of these coupons I would put a 294 00:11:56,750 --> 00:11:54,480 different bacterium and these are 295 00:11:58,970 --> 00:11:56,760 examples just with a felt tip Panda so 296 00:12:01,670 --> 00:11:58,980 they'll show up oh but if you could 297 00:12:04,730 --> 00:12:01,680 think the blue coupons are one species 298 00:12:07,009 --> 00:12:04,740 of bacterium uh and then the red is a 299 00:12:10,130 --> 00:12:07,019 different species I would place this 300 00:12:12,110 --> 00:12:10,140 inside the Mars chamber allow the 301 00:12:15,410 --> 00:12:12,120 ultraviolet irradiation to come down 302 00:12:18,769 --> 00:12:15,420 strike those samples and it's just 303 00:12:21,829 --> 00:12:18,779 looking for survival uh how well Can the 304 00:12:24,410 --> 00:12:21,839 bacteria and fungi survive just exposure 305 00:12:28,430 --> 00:12:24,420 to that solar ultraviolet or radiation 306 00:12:32,389 --> 00:12:28,440 and it turns out uh that if the 307 00:12:35,810 --> 00:12:32,399 bacterian fungi are exposed out on a 308 00:12:38,090 --> 00:12:35,820 flat surface or even if they're kind of 309 00:12:40,730 --> 00:12:38,100 shielded a little bit enough solar UV 310 00:12:43,610 --> 00:12:40,740 irradiation bounces around and Strikes 311 00:12:45,710 --> 00:12:43,620 them that populations as high as two or 312 00:12:48,350 --> 00:12:45,720 three million spores per square 313 00:12:51,889 --> 00:12:48,360 centimeter can be killed off within 314 00:12:55,190 --> 00:12:51,899 three or four hours on the surface of 315 00:12:57,170 --> 00:12:55,200 the Equator of Mars uh just within that 316 00:13:00,470 --> 00:12:57,180 first soul and if you didn't know a day 317 00:13:03,250 --> 00:13:00,480 on Mars is called the soul so in summary 318 00:13:05,810 --> 00:13:03,260 from a survival standpoint 319 00:13:07,970 --> 00:13:05,820 exposing bacteria and fungi just to the 320 00:13:10,190 --> 00:13:07,980 low pressure environment maybe we lose 321 00:13:11,569 --> 00:13:10,200 about 20 percent of a population over a 322 00:13:14,470 --> 00:13:11,579 few Souls 323 00:13:17,329 --> 00:13:14,480 if they're hit with solar UV irradiation 324 00:13:19,730 --> 00:13:17,339 uh they're killed off very very quickly 325 00:13:22,490 --> 00:13:19,740 but those are only two factors there's 326 00:13:24,769 --> 00:13:22,500 there's another 18 to 20 factors that 327 00:13:28,069 --> 00:13:24,779 have been discussed for the service of 328 00:13:30,310 --> 00:13:28,079 Mars High salts in the soil volatile 329 00:13:35,030 --> 00:13:30,320 oxidants in the soil and atmosphere 330 00:13:36,889 --> 00:13:35,040 solar UV radiation solar particle events 331 00:13:39,230 --> 00:13:36,899 from the Sun that might strike the 332 00:13:42,530 --> 00:13:39,240 planet and I won't go on but there's at 333 00:13:45,590 --> 00:13:42,540 least 20 biocidal factors all of them 334 00:13:48,769 --> 00:13:45,600 working synergistically to reduce the 335 00:13:51,949 --> 00:13:48,779 population of spores cells of both 336 00:13:54,110 --> 00:13:51,959 bacteria and fungi on the spacecraft 337 00:13:55,430 --> 00:13:54,120 well that's rather incredible and so I 338 00:13:56,870 --> 00:13:55,440 will come back to our Poll for our 339 00:13:57,949 --> 00:13:56,880 audience then with that too since you 340 00:13:59,509 --> 00:13:57,959 just said you know some of these spores 341 00:14:01,190 --> 00:13:59,519 will Thrive like you know you have this 342 00:14:02,569 --> 00:14:01,200 slow kind of process so something's 343 00:14:04,310 --> 00:14:02,579 dying off 344 00:14:05,930 --> 00:14:04,320 um when it comes to life on Earth there 345 00:14:07,550 --> 00:14:05,940 are different kind of ways for for 346 00:14:09,410 --> 00:14:07,560 organisms to adapt to the radiation 347 00:14:10,610 --> 00:14:09,420 environment for instance in different 348 00:14:12,110 --> 00:14:10,620 places 349 00:14:14,629 --> 00:14:12,120 um E coli for instance can actually take 350 00:14:15,769 --> 00:14:14,639 a much larger dose than humans can 351 00:14:18,650 --> 00:14:15,779 um I think the last I read was something 352 00:14:20,629 --> 00:14:18,660 like 200 to 700 Grays was kind of a an 353 00:14:22,009 --> 00:14:20,639 average decent dose that E coli could 354 00:14:24,470 --> 00:14:22,019 still thrive in which is higher than the 355 00:14:26,389 --> 00:14:24,480 Martian surface for our Poll for 356 00:14:27,949 --> 00:14:26,399 dinococcus radio durans which by the way 357 00:14:30,410 --> 00:14:27,959 still holds the record that we know of 358 00:14:32,269 --> 00:14:30,420 for a radiation dose it looks like most 359 00:14:35,629 --> 00:14:32,279 of our respondents said either a hundred 360 00:14:38,269 --> 00:14:35,639 Grays or a thousand Grays but indeed the 361 00:14:41,449 --> 00:14:38,279 answer was five thousand plus they can 362 00:14:43,730 --> 00:14:41,459 take a dose well over 5 000 times what 363 00:14:46,129 --> 00:14:43,740 would get a human sick and still Thrive 364 00:14:48,650 --> 00:14:46,139 even tartar grades have been put into 4 365 00:14:50,870 --> 00:14:48,660 000 gray dose environments and have 366 00:14:51,889 --> 00:14:50,880 survived and so this is you know that 367 00:14:53,870 --> 00:14:51,899 those are very high radiation 368 00:14:56,389 --> 00:14:53,880 environments we put dinocoxious radio 369 00:14:59,030 --> 00:14:56,399 durans into the the cooling fluid from 370 00:15:02,329 --> 00:14:59,040 from nuclear radioactive uh Radioactive 371 00:15:04,730 --> 00:15:02,339 uh uh plants for energy and they've 372 00:15:06,290 --> 00:15:04,740 survived inside of that cooling fluid 373 00:15:08,389 --> 00:15:06,300 um and so you know these things can take 374 00:15:10,009 --> 00:15:08,399 a pretty hard blast but then you start 375 00:15:11,569 --> 00:15:10,019 adding in all these other conditions 376 00:15:13,670 --> 00:15:11,579 just like Dr sugar mentioned you know 377 00:15:16,009 --> 00:15:13,680 with the low pressure and then salts and 378 00:15:17,629 --> 00:15:16,019 the soil different gas compositions dust 379 00:15:19,730 --> 00:15:17,639 and those are the things that we need to 380 00:15:21,590 --> 00:15:19,740 study together in a systems approach and 381 00:15:24,110 --> 00:15:21,600 so it's really cool your lab is doing 382 00:15:26,030 --> 00:15:24,120 that with this Mars chamber have there 383 00:15:28,069 --> 00:15:26,040 been any really kind of exciting or 384 00:15:30,110 --> 00:15:28,079 surprising results maybe from that 385 00:15:33,110 --> 00:15:30,120 research with the Mars chamber 386 00:15:35,210 --> 00:15:33,120 uh well I I can't go and answer that 387 00:15:37,850 --> 00:15:35,220 question until I make one comment on 388 00:15:39,829 --> 00:15:37,860 dinococcus radio durans I was sort of 389 00:15:42,170 --> 00:15:39,839 shuffling around there just a moment ago 390 00:15:44,509 --> 00:15:42,180 because I literally downloaded a paper 391 00:15:47,509 --> 00:15:44,519 this morning that talked about radio 392 00:15:49,810 --> 00:15:47,519 dinococcus radio durans surviving 393 00:15:54,050 --> 00:15:49,820 exposure up to 394 00:15:56,210 --> 00:15:54,060 120 kilograms now a kilogram is a 395 00:16:00,350 --> 00:15:56,220 thousand gray so that would be a hundred 396 00:16:03,949 --> 00:16:00,360 and twenty thousand Grays the reason why 397 00:16:06,829 --> 00:16:03,959 humans have sort of like a buy I have a 398 00:16:10,310 --> 00:16:06,839 a very severe reaction to even extremely 399 00:16:12,710 --> 00:16:10,320 low dosage of of radiation is that that 400 00:16:15,310 --> 00:16:12,720 radiation is exposing billions and 401 00:16:17,870 --> 00:16:15,320 billions of cells in our body 402 00:16:20,750 --> 00:16:17,880 whereas a bacterium is a single cell 403 00:16:23,569 --> 00:16:20,760 sitting out on a Surface so you have to 404 00:16:26,329 --> 00:16:23,579 get the intensity dramatically higher to 405 00:16:28,730 --> 00:16:26,339 get that same lethality it's just a body 406 00:16:30,889 --> 00:16:28,740 mass issue that's that's uh the 407 00:16:33,650 --> 00:16:30,899 difference between human survival and a 408 00:16:35,389 --> 00:16:33,660 bacterium surviving so I digress what 409 00:16:37,129 --> 00:16:35,399 was the question well that's a very good 410 00:16:39,110 --> 00:16:37,139 point so we we did see that paper as 411 00:16:40,610 --> 00:16:39,120 well and planning for this episode and 412 00:16:43,009 --> 00:16:40,620 it was just so shocking that the 413 00:16:45,350 --> 00:16:43,019 radiation dose was that High 414 00:16:46,550 --> 00:16:45,360 um that we just did 5000 plus 415 00:16:47,990 --> 00:16:46,560 um you know and so there's some more 416 00:16:51,170 --> 00:16:48,000 results to support that conclusion but 417 00:16:52,790 --> 00:16:51,180 there's a rather incredible conclusion 418 00:16:54,829 --> 00:16:52,800 um but I think you know my big question 419 00:16:57,050 --> 00:16:54,839 using this Mars chamber you know really 420 00:16:59,150 --> 00:16:57,060 having kind of the systems approach now 421 00:17:00,470 --> 00:16:59,160 to multiple different scenarios you know 422 00:17:03,230 --> 00:17:00,480 these these five different conditions 423 00:17:05,390 --> 00:17:03,240 that we made experience on Mars right 424 00:17:07,370 --> 00:17:05,400 had any really you know surprising or 425 00:17:09,770 --> 00:17:07,380 drastic results that kind of changed how 426 00:17:14,150 --> 00:17:09,780 you're thinking about life on Mars 427 00:17:16,909 --> 00:17:14,160 yes uh oh about 10 12 years ago I 428 00:17:19,610 --> 00:17:16,919 started research moving from survival 429 00:17:23,090 --> 00:17:19,620 which is in my view survival is a term 430 00:17:26,350 --> 00:17:23,100 that I would use for dormant or inactive 431 00:17:28,909 --> 00:17:26,360 cells or spores persisting through time 432 00:17:31,250 --> 00:17:28,919 uh they don't have to replicate they're 433 00:17:34,130 --> 00:17:31,260 not taking in nutrients metabolizing and 434 00:17:36,890 --> 00:17:34,140 dividing so survival could just be a 435 00:17:39,169 --> 00:17:36,900 dormant cell lasting a long time 436 00:17:41,210 --> 00:17:39,179 I did a lot of work with survival under 437 00:17:42,950 --> 00:17:41,220 in the Mars chamber and under UV 438 00:17:44,870 --> 00:17:42,960 irradiation and vacuum and temperature 439 00:17:46,789 --> 00:17:44,880 extremes and then I started getting 440 00:17:48,710 --> 00:17:46,799 interested in bacterial growth 441 00:17:50,770 --> 00:17:48,720 metabolism and growth 442 00:17:53,210 --> 00:17:50,780 and so I was interested in in 443 00:17:56,630 --> 00:17:53,220 determining at seven millibars of 444 00:17:58,490 --> 00:17:56,640 pressure at a zero centigrade and with 445 00:18:01,730 --> 00:17:58,500 enough liquid water present to keep 446 00:18:04,970 --> 00:18:01,740 cells hydrated could any terrestrial 447 00:18:07,850 --> 00:18:04,980 bacteria actually metabolize and grow 448 00:18:10,190 --> 00:18:07,860 under these Martian surface conditions 449 00:18:13,010 --> 00:18:10,200 and I did experiment after experiment 450 00:18:15,650 --> 00:18:13,020 bacterium after bacterium and I 451 00:18:17,810 --> 00:18:15,660 literally was just ready to say okay 452 00:18:19,490 --> 00:18:17,820 I've tried to write a paper that says 453 00:18:23,690 --> 00:18:19,500 nothing grew under Martian conditions 454 00:18:26,750 --> 00:18:23,700 and the very last bacterium I tested 455 00:18:29,150 --> 00:18:26,760 actually grew under the low pressure low 456 00:18:32,090 --> 00:18:29,160 temperature and gas composition in the 457 00:18:34,549 --> 00:18:32,100 assays so it was a really pleasant 458 00:18:36,770 --> 00:18:34,559 surprise and once you establish a 459 00:18:38,690 --> 00:18:36,780 protocol that confirmed that this 460 00:18:41,570 --> 00:18:38,700 bacterium's name by the way is serratia 461 00:18:44,630 --> 00:18:41,580 liquidations it's a non-spore forming 462 00:18:47,450 --> 00:18:44,640 bacteria found in spacecraft and on 463 00:18:50,150 --> 00:18:47,460 surfaces of Hardware mainly Hardware 464 00:18:52,330 --> 00:18:50,160 associated with human activity like on 465 00:18:55,730 --> 00:18:52,340 the space station or Apollo spacecraft 466 00:18:58,210 --> 00:18:55,740 Skylab things like that but in any event 467 00:19:00,710 --> 00:18:58,220 once you have I have the basic protocol 468 00:19:02,510 --> 00:19:00,720 that I know something will grow under 469 00:19:04,970 --> 00:19:02,520 motion distance then we could start 470 00:19:08,090 --> 00:19:04,980 going through large numbers of bacteria 471 00:19:11,750 --> 00:19:08,100 a lot faster and to date we've 472 00:19:15,950 --> 00:19:11,760 identified approximately 30 bacteria but 473 00:19:17,890 --> 00:19:15,960 no fungi so far that can grow metabolize 474 00:19:20,390 --> 00:19:17,900 and grow under low pressure 475 00:19:23,029 --> 00:19:20,400 hydrated conditions similar to the 476 00:19:25,970 --> 00:19:23,039 surface of Mars so that's that just with 477 00:19:28,070 --> 00:19:25,980 one bacterium one last test changed my 478 00:19:31,010 --> 00:19:28,080 whole perspective on what we what might 479 00:19:32,570 --> 00:19:31,020 be possible on the surface of Mars wow 480 00:19:34,070 --> 00:19:32,580 yeah you know instead of being Eureka it 481 00:19:35,810 --> 00:19:34,080 was a huh I didn't I didn't think that 482 00:19:37,970 --> 00:19:35,820 would happen kind of moment I love that 483 00:19:39,590 --> 00:19:37,980 so much and that kind of also then it's 484 00:19:41,690 --> 00:19:39,600 important for my next topic I kind of 485 00:19:43,549 --> 00:19:41,700 want to discuss here is the idea of 486 00:19:44,750 --> 00:19:43,559 forward contamination 487 00:19:46,909 --> 00:19:44,760 um you know there's a whole department 488 00:19:48,110 --> 00:19:46,919 an office at Nasa that does planetary 489 00:19:49,730 --> 00:19:48,120 protection 490 00:19:51,130 --> 00:19:49,740 um figuring out you know how do we you 491 00:19:53,570 --> 00:19:51,140 know just not not only avoid 492 00:19:55,250 --> 00:19:53,580 contaminating Earth with possible things 493 00:19:57,230 --> 00:19:55,260 from elsewhere but how do we avoid 494 00:19:59,750 --> 00:19:57,240 taking Earth life and contaminating 495 00:20:01,730 --> 00:19:59,760 other places and you know no matter how 496 00:20:04,070 --> 00:20:01,740 much we try to clean our cleanest clean 497 00:20:06,169 --> 00:20:04,080 rooms are still going to have some some 498 00:20:08,510 --> 00:20:06,179 biology we're still going to send some 499 00:20:10,250 --> 00:20:08,520 things with our Rovers and when we send 500 00:20:12,350 --> 00:20:10,260 humans it gets even more crazy because 501 00:20:14,210 --> 00:20:12,360 you know we humans have a microbiome and 502 00:20:16,130 --> 00:20:14,220 we're shedding off organisms all the 503 00:20:18,110 --> 00:20:16,140 time not just our own cells but other 504 00:20:19,430 --> 00:20:18,120 organisms with us 505 00:20:21,289 --> 00:20:19,440 um and so you know we'll have this this 506 00:20:23,330 --> 00:20:21,299 moment when we get to Mars when humans 507 00:20:25,430 --> 00:20:23,340 start exploring especially where you 508 00:20:27,529 --> 00:20:25,440 know we are you know putting out Earthly 509 00:20:28,669 --> 00:20:27,539 organisms on the surface of Mars and I 510 00:20:30,770 --> 00:20:28,679 know you've done some work in that 511 00:20:33,529 --> 00:20:30,780 looking at at how we're going to have 512 00:20:34,610 --> 00:20:33,539 some issues with forward contamination 513 00:20:36,590 --> 00:20:34,620 um I wonder if you can speak to that 514 00:20:38,450 --> 00:20:36,600 issue with how we might you know try to 515 00:20:40,310 --> 00:20:38,460 avoid you know dumping a bunch of 516 00:20:42,110 --> 00:20:40,320 organisms on mars or or can we even 517 00:20:44,810 --> 00:20:42,120 avoid that at all 518 00:20:46,370 --> 00:20:44,820 well uh we have to step back a little 519 00:20:47,990 --> 00:20:46,380 bit before we go to that specific 520 00:20:50,930 --> 00:20:48,000 question 521 00:20:53,090 --> 00:20:50,940 um look at any image of a Rover like the 522 00:20:56,750 --> 00:20:53,100 perseverance we're over right now or 523 00:20:59,330 --> 00:20:56,760 curiosity uh and you see this structure 524 00:21:01,789 --> 00:20:59,340 these very fairly large structures with 525 00:21:03,650 --> 00:21:01,799 all these little nooks and crannies in 526 00:21:06,289 --> 00:21:03,660 it uh 527 00:21:08,510 --> 00:21:06,299 but if everybody's got to kind of keep 528 00:21:10,669 --> 00:21:08,520 in mind that even if there are bacteria 529 00:21:13,549 --> 00:21:10,679 and maybe a few fungi and a few of the 530 00:21:16,850 --> 00:21:13,559 prokaryotes called archaea if those are 531 00:21:19,789 --> 00:21:16,860 all present on the surface that surface 532 00:21:23,289 --> 00:21:19,799 is being immediately bathed on as soon 533 00:21:26,870 --> 00:21:23,299 as it lands in an extremely strong 534 00:21:30,190 --> 00:21:26,880 ultraviolet radiation environment and 535 00:21:32,750 --> 00:21:30,200 photons of light are incredibly small 536 00:21:35,870 --> 00:21:32,760 scientific Community doesn't even have a 537 00:21:38,810 --> 00:21:35,880 consensus on how big they are the best 538 00:21:42,830 --> 00:21:38,820 eye estimate I have is 10 to the minus 539 00:21:44,930 --> 00:21:42,840 18 centimeters as the upper limit of how 540 00:21:47,870 --> 00:21:44,940 big they might be it's believed to be 541 00:21:50,210 --> 00:21:47,880 much smaller so these incredibly tiny 542 00:21:53,210 --> 00:21:50,220 little energy packets can get into 543 00:21:55,190 --> 00:21:53,220 almost every pit every crack Bounce 544 00:21:58,490 --> 00:21:55,200 Around bounce off of one surface to get 545 00:22:01,490 --> 00:21:58,500 into a shaded area and I've been doing 546 00:22:03,230 --> 00:22:01,500 modeling several papers out working with 547 00:22:07,669 --> 00:22:03,240 other colleagues to try to push the 548 00:22:10,490 --> 00:22:07,679 modeling and the UV environment alone is 549 00:22:12,890 --> 00:22:10,500 so strong on the surface of Mars most of 550 00:22:16,190 --> 00:22:12,900 these surfaces of spacecraft and rovers 551 00:22:19,070 --> 00:22:16,200 are being sterilized within a few souls 552 00:22:21,049 --> 00:22:19,080 for the upper deck and maybe a few 553 00:22:24,049 --> 00:22:21,059 months for the stuff that's underneath 554 00:22:27,190 --> 00:22:24,059 on the belly of the spacecraft so it's 555 00:22:31,310 --> 00:22:27,200 not the bacteria are not lasting for 556 00:22:33,830 --> 00:22:31,320 Generations uh it's very likely that the 557 00:22:36,470 --> 00:22:33,840 microbiota on these Rovers is being 558 00:22:38,510 --> 00:22:36,480 inactivated very quickly okay so we 559 00:22:41,510 --> 00:22:38,520 pause there now let's look at human 560 00:22:44,450 --> 00:22:41,520 missions human missions yes humans will 561 00:22:46,789 --> 00:22:44,460 have a much richer biome that they will 562 00:22:48,649 --> 00:22:46,799 bring with them but that biome is 563 00:22:51,470 --> 00:22:48,659 contained within a habitat 564 00:22:53,930 --> 00:22:51,480 and when they go out and do an Eva it's 565 00:22:55,250 --> 00:22:53,940 can that microbiome is contained within 566 00:22:57,890 --> 00:22:55,260 their spacesuits 567 00:23:00,770 --> 00:22:57,900 so when an astronaut just can think of 568 00:23:02,690 --> 00:23:00,780 this as uh just conceptually they may 569 00:23:06,350 --> 00:23:02,700 have contamination on the outside of 570 00:23:08,990 --> 00:23:06,360 their uh spacesuit they walk out into 571 00:23:11,630 --> 00:23:09,000 that incredibly Rich intense UV 572 00:23:14,149 --> 00:23:11,640 environment on Mars we're not going to 573 00:23:17,149 --> 00:23:14,159 do Evas at night because of temperature 574 00:23:19,190 --> 00:23:17,159 issues it's and lighting issues so it'll 575 00:23:20,750 --> 00:23:19,200 almost always be done in the daytime 576 00:23:24,230 --> 00:23:20,760 and that 577 00:23:27,289 --> 00:23:24,240 the data from multiple Labs supports a 578 00:23:30,529 --> 00:23:27,299 conclusion that most of the microbiomes 579 00:23:33,169 --> 00:23:30,539 on the outside surfaces of objects on 580 00:23:34,310 --> 00:23:33,179 Mars can be sterilized within a few 581 00:23:37,669 --> 00:23:34,320 hours 582 00:23:40,549 --> 00:23:37,679 on one Soul so if an astronaut goes out 583 00:23:42,049 --> 00:23:40,559 for eight hours nine hours of an Eva on 584 00:23:44,090 --> 00:23:42,059 the surface of Mars 585 00:23:46,490 --> 00:23:44,100 his outside or her outside of that 586 00:23:48,710 --> 00:23:46,500 spacesuit might be sterilized during the 587 00:23:51,649 --> 00:23:48,720 middle of it by the time he gets to the 588 00:23:55,010 --> 00:23:51,659 four hour mark in uh 589 00:23:57,590 --> 00:23:55,020 in his Eva so I'm not that concerned 590 00:24:01,010 --> 00:23:57,600 we're going to overtly ruin the surface 591 00:24:04,130 --> 00:24:01,020 and contaminate the service I think the 592 00:24:07,010 --> 00:24:04,140 more intriguing issue is how do we 593 00:24:07,850 --> 00:24:07,020 prevent bringing stuff back that might 594 00:24:12,230 --> 00:24:07,860 have 595 00:24:14,570 --> 00:24:12,240 a martian microbiota on it uh because we 596 00:24:15,710 --> 00:24:14,580 don't we don't know if one exists and if 597 00:24:17,510 --> 00:24:15,720 it does we don't know the 598 00:24:19,909 --> 00:24:17,520 characteristics of that life so that's 599 00:24:22,010 --> 00:24:19,919 back contamination I'm not so worried 600 00:24:24,409 --> 00:24:22,020 about forward contamination I'm a little 601 00:24:26,810 --> 00:24:24,419 bit more interested now on what might 602 00:24:29,750 --> 00:24:26,820 happen bringing a martian microbiota 603 00:24:31,190 --> 00:24:29,760 back to Earth if it's present which we 604 00:24:33,649 --> 00:24:31,200 don't it's a very good point we're going 605 00:24:34,669 --> 00:24:33,659 to do Mars sample return soon 606 00:24:37,130 --> 00:24:34,679 um starting with the samples being 607 00:24:39,169 --> 00:24:37,140 cached right now from perseverance but 608 00:24:40,190 --> 00:24:39,179 very likely in the not too distant 609 00:24:41,690 --> 00:24:40,200 future 610 00:24:43,430 --> 00:24:41,700 um maybe not you know tomorrow but in 611 00:24:45,289 --> 00:24:43,440 the next you know a couple of decades 612 00:24:47,149 --> 00:24:45,299 once humans do go to Mars we'll most 613 00:24:48,710 --> 00:24:47,159 likely bring humans back from Mars too 614 00:24:49,669 --> 00:24:48,720 eventually 615 00:24:51,770 --> 00:24:49,679 um and they'll probably bring some 616 00:24:54,110 --> 00:24:51,780 samples back with them and so that 617 00:24:55,430 --> 00:24:54,120 possibility is really important I do 618 00:24:57,649 --> 00:24:55,440 want to bring up another Twitter poll 619 00:24:58,310 --> 00:24:57,659 that we had uh through the NASA oh go 620 00:24:59,930 --> 00:24:58,320 ahead 621 00:25:02,870 --> 00:24:59,940 I was just going to say one other 622 00:25:03,890 --> 00:25:02,880 important point though is that 623 00:25:06,409 --> 00:25:03,900 um 624 00:25:09,950 --> 00:25:06,419 absence of data 625 00:25:12,350 --> 00:25:09,960 does not confirm anything 626 00:25:14,930 --> 00:25:12,360 so as a scientist you have to just say 627 00:25:17,390 --> 00:25:14,940 there's no data to say this 628 00:25:21,649 --> 00:25:17,400 now don't conclude something from that 629 00:25:23,510 --> 00:25:21,659 lack of data it's just no data and so 630 00:25:26,149 --> 00:25:23,520 you keep your options open you think of 631 00:25:28,430 --> 00:25:26,159 all the possibilities but it is it is 632 00:25:31,250 --> 00:25:28,440 equally plausible that the surface of 633 00:25:32,750 --> 00:25:31,260 Mars and all the regolith is literally 634 00:25:40,730 --> 00:25:32,760 sterilized 635 00:25:44,510 --> 00:25:40,740 those 20 volocital factors that is as 636 00:25:48,130 --> 00:25:44,520 equally plausible as some life form that 637 00:25:52,370 --> 00:25:48,140 evolved three billion years ago actually 638 00:25:54,490 --> 00:25:52,380 uh persisting in learning how to to grow 639 00:25:58,970 --> 00:25:54,500 metabolize and evolve on the surface 640 00:26:00,830 --> 00:25:58,980 equal no data so that's we we don't jump 641 00:26:03,950 --> 00:26:00,840 to either one of those as a scientist 642 00:26:05,110 --> 00:26:03,960 and the same thing should hold for the 643 00:26:10,970 --> 00:26:05,120 uh 644 00:26:12,230 --> 00:26:10,980 are going to help decide the protocols 645 00:26:13,549 --> 00:26:12,240 for both forward and backward 646 00:26:15,529 --> 00:26:13,559 contamination 647 00:26:17,810 --> 00:26:15,539 absolutely 648 00:26:20,090 --> 00:26:17,820 um so I will say uh of our audience we 649 00:26:22,730 --> 00:26:20,100 we did ask through Twitter uh through 650 00:26:24,289 --> 00:26:22,740 the at Nasa astrobio Twitter account we 651 00:26:25,730 --> 00:26:24,299 we asked people what they thought would 652 00:26:27,890 --> 00:26:25,740 be the most important obstacle that 653 00:26:29,930 --> 00:26:27,900 Earth life might face on Mars with 654 00:26:33,049 --> 00:26:29,940 options like radiation low gravity low 655 00:26:34,789 --> 00:26:33,059 temperature the very dry conditions and 656 00:26:36,649 --> 00:26:34,799 you know overwhelmingly people said 657 00:26:38,149 --> 00:26:36,659 radiation 658 00:26:39,590 --> 00:26:38,159 um was the largest factor they think 659 00:26:41,510 --> 00:26:39,600 would be an issue out of these other 660 00:26:43,430 --> 00:26:41,520 possible factors 661 00:26:46,370 --> 00:26:43,440 um so do you think you know if there was 662 00:26:48,590 --> 00:26:46,380 Martian biota in the distant past that 663 00:26:50,630 --> 00:26:48,600 may still be alive or went extinct long 664 00:26:52,970 --> 00:26:50,640 ago but if there were some possibility 665 00:26:54,769 --> 00:26:52,980 for there to be life on Mars do you 666 00:26:56,990 --> 00:26:54,779 think we'd have to get a sample from 667 00:26:59,630 --> 00:26:57,000 deeper down under the surface to avoid 668 00:27:01,970 --> 00:26:59,640 the radiation to really find any fines 669 00:27:04,130 --> 00:27:01,980 of any signs of possible past or present 670 00:27:06,289 --> 00:27:04,140 life that's Martian life 671 00:27:08,930 --> 00:27:06,299 uh okay so there's two types of 672 00:27:11,110 --> 00:27:08,940 radiation the radiation the way you're 673 00:27:14,450 --> 00:27:11,120 using the word refers to probably 674 00:27:17,990 --> 00:27:14,460 ionizing radiation this is like x-rays 675 00:27:21,590 --> 00:27:18,000 gamma rays protons helium atoms 676 00:27:25,149 --> 00:27:21,600 Etc uh very high energies uh and they 677 00:27:29,990 --> 00:27:25,159 can ionize molecules there's also the 678 00:27:32,570 --> 00:27:30,000 electromagnetic radiation of UV light so 679 00:27:34,730 --> 00:27:32,580 UV light in my view is the strongest 680 00:27:38,149 --> 00:27:34,740 biocidal Factor on the surface of Mars 681 00:27:39,370 --> 00:27:38,159 followed by desiccation low pressure and 682 00:27:41,710 --> 00:27:39,380 low temperature 683 00:27:44,810 --> 00:27:41,720 uh the 684 00:27:48,890 --> 00:27:44,820 ionizing radiation like gamma rays 685 00:27:51,409 --> 00:27:48,900 x-rays that dosage is relatively benign 686 00:27:53,870 --> 00:27:51,419 from the standpoint of bacterial 687 00:27:56,570 --> 00:27:53,880 survival based on Earth bacterial 688 00:27:58,190 --> 00:27:56,580 survival under various intensities of 689 00:28:02,110 --> 00:27:58,200 ionizing radiation 690 00:28:05,630 --> 00:28:02,120 so I think the radiation environment for 691 00:28:07,010 --> 00:28:05,640 inactivation on the surface is this UV 692 00:28:09,649 --> 00:28:07,020 irradiation 693 00:28:12,470 --> 00:28:09,659 the hard radiation or ionizing radiation 694 00:28:14,269 --> 00:28:12,480 is probably only a minor Factor unless 695 00:28:15,470 --> 00:28:14,279 you're talking about geological time 696 00:28:17,210 --> 00:28:15,480 periods 697 00:28:18,590 --> 00:28:17,220 intriguing yeah that's really good to 698 00:28:20,390 --> 00:28:18,600 know and a good reminder for our 699 00:28:22,610 --> 00:28:20,400 audience too about the processes that 700 00:28:25,310 --> 00:28:22,620 affect you know living things on Mars as 701 00:28:26,930 --> 00:28:25,320 well as possible things like Organics 702 00:28:28,850 --> 00:28:26,940 um and so we I do want to jump to our 703 00:28:30,590 --> 00:28:28,860 audience uh questions here as soon as I 704 00:28:31,610 --> 00:28:30,600 can but there are two more quick things 705 00:28:33,710 --> 00:28:31,620 I do want to bring up about your 706 00:28:36,470 --> 00:28:33,720 research that you've been working on uh 707 00:28:39,169 --> 00:28:36,480 either now or in the past and one has to 708 00:28:41,570 --> 00:28:39,179 do with with UV on the surface of Mars 709 00:28:44,029 --> 00:28:41,580 you've been doing some work recently on 710 00:28:46,789 --> 00:28:44,039 studying the processes of forming and 711 00:28:48,409 --> 00:28:46,799 breaking down methane at the surface of 712 00:28:49,789 --> 00:28:48,419 Mars and you know for those who are 713 00:28:51,350 --> 00:28:49,799 watching they might be aware that you 714 00:28:53,930 --> 00:28:51,360 know some years ago we had the first 715 00:28:56,510 --> 00:28:53,940 potential detections of methane in large 716 00:28:58,070 --> 00:28:56,520 plumes the the Martian surface and now 717 00:29:00,110 --> 00:28:58,080 more recently we've had more 718 00:29:01,430 --> 00:29:00,120 confirmation of methane at the surface 719 00:29:03,470 --> 00:29:01,440 of Mars 720 00:29:04,909 --> 00:29:03,480 um so so what has your research been in 721 00:29:06,350 --> 00:29:04,919 looking at this production and 722 00:29:07,610 --> 00:29:06,360 destructive process when it comes to 723 00:29:10,190 --> 00:29:07,620 methane 724 00:29:12,350 --> 00:29:10,200 all right so a number of years ago I 725 00:29:15,909 --> 00:29:12,360 worked with several other scientists to 726 00:29:18,169 --> 00:29:15,919 explore how the ultraviolet irradiation 727 00:29:19,130 --> 00:29:18,179 environment that hits the surface of 728 00:29:21,409 --> 00:29:19,140 Mars 729 00:29:25,010 --> 00:29:21,419 can't is it energetic enough does it 730 00:29:28,330 --> 00:29:25,020 have enough energy per photon to break 731 00:29:30,350 --> 00:29:28,340 Bonds in a methane in a organic compound 732 00:29:33,950 --> 00:29:30,360 releasing methane 733 00:29:37,010 --> 00:29:33,960 and it turns out that it does uh the 734 00:29:40,310 --> 00:29:37,020 exact mechanism uh if for which that 735 00:29:44,389 --> 00:29:40,320 that process occurs is not really worked 736 00:29:48,590 --> 00:29:44,399 out but it clearly our research clearly 737 00:29:51,730 --> 00:29:48,600 demonstrated uh that the ultraviolet 738 00:29:55,010 --> 00:29:51,740 irradiation that reaches the surface can 739 00:29:56,389 --> 00:29:55,020 cleave or break down Organics in the 740 00:29:59,330 --> 00:29:56,399 surface environment and in the 741 00:30:02,570 --> 00:29:59,340 atmosphere releasing methane what's 742 00:30:06,950 --> 00:30:02,580 intriguing is that the Curiosity Rover 743 00:30:10,490 --> 00:30:06,960 with the uh an instrument called the MSL 744 00:30:13,430 --> 00:30:10,500 um I'm sorry called Sam Dash TLS which 745 00:30:16,310 --> 00:30:13,440 is an organic detection instrument with 746 00:30:18,590 --> 00:30:16,320 a specific tunable laser that it can 747 00:30:22,130 --> 00:30:18,600 detect methane very very small levels of 748 00:30:23,990 --> 00:30:22,140 methane it had a has had there's a 749 00:30:26,149 --> 00:30:24,000 number of papers published with this 750 00:30:28,450 --> 00:30:26,159 detection down in the parts per billion 751 00:30:32,049 --> 00:30:28,460 range so 752 00:30:35,450 --> 00:30:32,059 2017 15 years ago we had data from Earth 753 00:30:37,850 --> 00:30:35,460 telescopes of large plumes of methane in 754 00:30:41,029 --> 00:30:37,860 the Martian atmosphere followed with 755 00:30:43,789 --> 00:30:41,039 some skepticism by the community then 756 00:30:46,789 --> 00:30:43,799 the MSL rover Curiosity started 757 00:30:51,110 --> 00:30:46,799 detecting it and showing spikes in it in 758 00:30:53,750 --> 00:30:51,120 methane but then the trace gas Orbiter 759 00:30:58,430 --> 00:30:53,760 which is an Esa instrument or a 760 00:31:00,830 --> 00:30:58,440 spacecraft it is now completely gone 761 00:31:03,610 --> 00:31:00,840 negative on that methane story it cannot 762 00:31:06,350 --> 00:31:03,620 detect it and it's setting extremely low 763 00:31:09,190 --> 00:31:06,360 levels for methane in the bulk mesh and 764 00:31:11,350 --> 00:31:09,200 atmosphere so what I think is happening 765 00:31:14,630 --> 00:31:11,360 based on just the literature available 766 00:31:17,990 --> 00:31:14,640 is that the methane concentrations that 767 00:31:20,450 --> 00:31:18,000 are being detected by the Mars rover 768 00:31:23,690 --> 00:31:20,460 Curiosity could be very close to the 769 00:31:26,330 --> 00:31:23,700 ground maybe only up to a few tens of 770 00:31:27,830 --> 00:31:26,340 meters to a few hundreds of meters and 771 00:31:30,830 --> 00:31:27,840 then those are dissipated through the 772 00:31:33,430 --> 00:31:30,840 day and when the methane is diluted out 773 00:31:35,930 --> 00:31:33,440 in the bulk atmosphere 774 00:31:38,990 --> 00:31:35,940 then the trace gas Orbiter is having 775 00:31:41,630 --> 00:31:39,000 trouble seeing that methane now that's 776 00:31:43,970 --> 00:31:41,640 an open area of study but if we can 777 00:31:47,029 --> 00:31:43,980 confirm that methane exists 778 00:31:50,889 --> 00:31:47,039 we it has to constantly be resupplied 779 00:31:53,990 --> 00:31:50,899 because the solar UV 780 00:31:56,690 --> 00:31:54,000 breaks down methane which is what my 781 00:31:59,210 --> 00:31:56,700 research and others have shown uh so 782 00:32:01,190 --> 00:31:59,220 it's a very interesting topic because if 783 00:32:03,470 --> 00:32:01,200 it's there and we can confirm it it 784 00:32:06,430 --> 00:32:03,480 could be a biogenic signature or bio 785 00:32:10,130 --> 00:32:06,440 signature of some Subterranean 786 00:32:12,590 --> 00:32:10,140 methanogenic Community it might be three 787 00:32:16,549 --> 00:32:12,600 four kilometers down into the crust of 788 00:32:19,070 --> 00:32:16,559 Mars uh but if it's present it'll those 789 00:32:21,230 --> 00:32:19,080 those will seep out eventually and and 790 00:32:23,029 --> 00:32:21,240 that's what we might be seeing but I'm 791 00:32:25,250 --> 00:32:23,039 starting to get into speculation here so 792 00:32:27,529 --> 00:32:25,260 sticking with just the data we have 793 00:32:29,510 --> 00:32:27,539 these versus big plumes that are local 794 00:32:31,610 --> 00:32:29,520 detection now the trace gas Orbiter is 795 00:32:33,049 --> 00:32:31,620 saying well not so fast maybe it's not 796 00:32:35,090 --> 00:32:33,059 there 797 00:32:36,409 --> 00:32:35,100 yeah it's rather incredible and you know 798 00:32:37,909 --> 00:32:36,419 your career you've done so many 799 00:32:40,909 --> 00:32:37,919 different things and I love that about 800 00:32:42,610 --> 00:32:40,919 astrobiology you know myself everyone I 801 00:32:44,990 --> 00:32:42,620 speak to in the realm of astrobiology 802 00:32:47,029 --> 00:32:45,000 hasn't just focused on one individual 803 00:32:49,010 --> 00:32:47,039 topic we've you know kind of touched all 804 00:32:50,750 --> 00:32:49,020 these different areas 805 00:32:52,370 --> 00:32:50,760 um and an astrobiology itself you know 806 00:32:55,070 --> 00:32:52,380 that's kind of crucial when it comes to 807 00:32:57,049 --> 00:32:55,080 understanding the nature of life and and 808 00:32:58,669 --> 00:32:57,059 one thing you worked on in the past was 809 00:33:01,250 --> 00:32:58,679 this thing called Dart uh the dust 810 00:33:02,690 --> 00:33:01,260 atmospheric recovery technology 811 00:33:04,669 --> 00:33:02,700 um for kind of looking at plant and 812 00:33:07,430 --> 00:33:04,679 human pathogens in dust plumes coming 813 00:33:09,110 --> 00:33:07,440 from Africa coming across the world and 814 00:33:11,269 --> 00:33:09,120 kind of coming here and that really 815 00:33:12,889 --> 00:33:11,279 sparked my own interests a lot I've been 816 00:33:14,450 --> 00:33:12,899 involved in the past 817 00:33:17,389 --> 00:33:14,460 um with some undergraduate level 818 00:33:19,669 --> 00:33:17,399 research and looking at uh aerobiology 819 00:33:22,070 --> 00:33:19,679 and how organisms are lofted into the 820 00:33:25,490 --> 00:33:22,080 atmosphere and move around a very good 821 00:33:27,529 --> 00:33:25,500 friend of mine David Smith NASA Ames he 822 00:33:29,750 --> 00:33:27,539 did research during his PhD looking at 823 00:33:31,549 --> 00:33:29,760 organisms being lofted from the Gobi 824 00:33:32,990 --> 00:33:31,559 desert and for eight days making their 825 00:33:34,789 --> 00:33:33,000 way across the world and kind of variety 826 00:33:37,190 --> 00:33:34,799 driving you know through the atmosphere 827 00:33:38,990 --> 00:33:37,200 to California and then you know we've 828 00:33:41,210 --> 00:33:39,000 now learned that the way the Amazon 829 00:33:44,330 --> 00:33:41,220 rainforest is being fertilized with 830 00:33:46,190 --> 00:33:44,340 phosphorus is from dust from the Saharan 831 00:33:48,169 --> 00:33:46,200 region of Africa being lofted and coming 832 00:33:51,710 --> 00:33:48,179 over here and it shows this beautiful 833 00:33:52,730 --> 00:33:51,720 connection of life on Earth but then you 834 00:33:53,630 --> 00:33:52,740 know you were also involved in this 835 00:33:55,789 --> 00:33:53,640 realm of kind of thinking about 836 00:33:57,889 --> 00:33:55,799 pathogens for plants and humans being 837 00:33:59,690 --> 00:33:57,899 lofted up as well I wonder if you could 838 00:34:01,549 --> 00:33:59,700 speak about you know that research what 839 00:34:03,769 --> 00:34:01,559 intrigued you about it and maybe kind of 840 00:34:05,330 --> 00:34:03,779 your vision for these things moving 841 00:34:10,669 --> 00:34:05,340 around the planet and this connectivity 842 00:34:13,550 --> 00:34:10,679 of Life yeah exactly the the planet uh 843 00:34:17,149 --> 00:34:13,560 the planet's atmosphere circulates 844 00:34:18,950 --> 00:34:17,159 pretty much everywhere so if in theory 845 00:34:21,290 --> 00:34:18,960 something is present in the Saharan 846 00:34:23,210 --> 00:34:21,300 Desert that will eventually be 847 00:34:25,970 --> 00:34:23,220 recirculated all over the planet 848 00:34:28,310 --> 00:34:25,980 eventually dropping or depositing out in 849 00:34:31,010 --> 00:34:28,320 the Arctic regions and the equatorial 850 00:34:34,490 --> 00:34:31,020 regions and the amount of dust being 851 00:34:36,829 --> 00:34:34,500 moved from the Sahara to North America 852 00:34:39,710 --> 00:34:36,839 is quite dramatic just in Florida every 853 00:34:42,829 --> 00:34:39,720 year 50 million metric tons of dust 854 00:34:44,690 --> 00:34:42,839 comes from Africa usually in the months 855 00:34:47,149 --> 00:34:44,700 of July middle of July through the 856 00:34:48,649 --> 00:34:47,159 middle of September but over in the 857 00:34:50,930 --> 00:34:48,659 Northwest this would be Northern 858 00:34:53,270 --> 00:34:50,940 California Oregon and Washington State 859 00:34:56,210 --> 00:34:53,280 up into British Columbia uh they're 860 00:34:58,550 --> 00:34:56,220 getting about another 60 or 70 million 861 00:35:01,730 --> 00:34:58,560 metric tons of dust coming from the 862 00:35:03,589 --> 00:35:01,740 Chinese the Gobi desert in China so 863 00:35:05,570 --> 00:35:03,599 there's a large volume of it bringing a 864 00:35:07,790 --> 00:35:05,580 lot of nutrients most of the fertility 865 00:35:10,130 --> 00:35:07,800 not only in the Amazon but in the 866 00:35:12,950 --> 00:35:10,140 islands in the Caribbean are due from 867 00:35:15,290 --> 00:35:12,960 the minerals that are coming from over 868 00:35:18,170 --> 00:35:15,300 geological time have come from the 869 00:35:21,109 --> 00:35:18,180 Saharan Desert So This Global movement 870 00:35:23,890 --> 00:35:21,119 is very interesting and there's a 871 00:35:26,569 --> 00:35:23,900 concept called emerging pathogens 872 00:35:29,329 --> 00:35:26,579 and there are a lot of institutes that 873 00:35:31,970 --> 00:35:29,339 study emerging pathogens a pathogen that 874 00:35:34,670 --> 00:35:31,980 was not present in one area suddenly 875 00:35:36,890 --> 00:35:34,680 becomes very active it could be a plant 876 00:35:39,410 --> 00:35:36,900 pathogen it could be an animal pathogen 877 00:35:41,210 --> 00:35:39,420 it could be a pathogen to fish or 878 00:35:43,609 --> 00:35:41,220 aquatic ecosystems or it could be a 879 00:35:45,109 --> 00:35:43,619 human pathogen so I just became as a 880 00:35:47,390 --> 00:35:45,119 plant pathologist I became very 881 00:35:49,250 --> 00:35:47,400 intrigued on whether or not plant 882 00:35:51,349 --> 00:35:49,260 pathogens were being moved around in 883 00:35:53,390 --> 00:35:51,359 these dust storms and the evidence 884 00:35:55,069 --> 00:35:53,400 pretty much supports that they are but 885 00:35:57,230 --> 00:35:55,079 we don't have it really kind of well 886 00:35:59,690 --> 00:35:57,240 characterized with the volume of those 887 00:36:02,450 --> 00:35:59,700 plant pathogens are and how much impact 888 00:36:04,250 --> 00:36:02,460 if any the plant pathogens are doing to 889 00:36:05,750 --> 00:36:04,260 the agricultural systems in these 890 00:36:07,730 --> 00:36:05,760 regions 891 00:36:09,290 --> 00:36:07,740 wow yeah and so for any of our audience 892 00:36:10,730 --> 00:36:09,300 who are watching who are interested in 893 00:36:13,130 --> 00:36:10,740 in this realm of research and 894 00:36:15,230 --> 00:36:13,140 microbiology and plant pathogens you 895 00:36:16,130 --> 00:36:15,240 know plant how plants function in 896 00:36:17,329 --> 00:36:16,140 general 897 00:36:18,829 --> 00:36:17,339 um there's so much to take from this 898 00:36:20,329 --> 00:36:18,839 that you can be an astrobiologist and 899 00:36:21,770 --> 00:36:20,339 work on so many of these different kinds 900 00:36:23,270 --> 00:36:21,780 of topics 901 00:36:24,349 --> 00:36:23,280 um I do want to move now I know that 902 00:36:25,910 --> 00:36:24,359 we're running out of time and so I want 903 00:36:28,130 --> 00:36:25,920 to move now to our faster than light 904 00:36:30,290 --> 00:36:28,140 segment this is where we just have some 905 00:36:31,670 --> 00:36:30,300 rapid fire questions 906 00:36:33,170 --> 00:36:31,680 um and so I just have a few that I want 907 00:36:34,609 --> 00:36:33,180 to ask you 908 00:36:36,530 --> 00:36:34,619 um to bring up some of these topics that 909 00:36:37,790 --> 00:36:36,540 are relevant to astrobiology and a lot 910 00:36:40,250 --> 00:36:37,800 of us kind of want to know from all the 911 00:36:43,430 --> 00:36:40,260 astrobiologists we speak to 912 00:36:44,930 --> 00:36:43,440 so the first question comes from Enrico 913 00:36:47,089 --> 00:36:44,940 Fermi when he when he first asked you 914 00:36:49,310 --> 00:36:47,099 know like where are they out there uh 915 00:36:51,410 --> 00:36:49,320 what is your favorite answer to fermi's 916 00:36:54,470 --> 00:36:51,420 question where are they 917 00:36:57,349 --> 00:36:54,480 oh fascinating I just read a book called 918 00:36:59,329 --> 00:36:57,359 where is everybody 919 00:37:02,690 --> 00:36:59,339 um and it's about trying to solve the 920 00:37:05,510 --> 00:37:02,700 Drake equation which was developed in an 921 00:37:06,410 --> 00:37:05,520 attempt to answer Enrico fermi's uh 922 00:37:09,230 --> 00:37:06,420 question 923 00:37:11,210 --> 00:37:09,240 and they gave 50 reasons why aliens 924 00:37:14,210 --> 00:37:11,220 haven't been in contact 50 plausible 925 00:37:18,290 --> 00:37:14,220 hypotheses of all of those I think the 926 00:37:21,829 --> 00:37:18,300 most reasonable is that the astronomical 927 00:37:25,430 --> 00:37:21,839 distance between Star systems are so 928 00:37:29,030 --> 00:37:25,440 incredibly huge that it is fundamentally 929 00:37:33,109 --> 00:37:29,040 impractical for aliens to travel between 930 00:37:35,270 --> 00:37:33,119 Star systems and just as an example uh 931 00:37:37,190 --> 00:37:35,280 Alpha Satori which is a cluster of three 932 00:37:38,829 --> 00:37:37,200 stars sort of in this very really 933 00:37:40,450 --> 00:37:38,839 incredible dance going on 934 00:37:44,990 --> 00:37:40,460 three-dimensional 935 00:37:48,710 --> 00:37:45,000 orbits that's 4.2 4.26 light years away 936 00:37:51,470 --> 00:37:48,720 current rocketry would take like 80 000 937 00:37:54,170 --> 00:37:51,480 years to have a spaceship reach the 938 00:37:57,109 --> 00:37:54,180 closest star so that I think is the best 939 00:37:59,109 --> 00:37:57,119 solution the distances are just way too 940 00:38:02,030 --> 00:37:59,119 big to have this easily happen 941 00:38:03,950 --> 00:38:02,040 absolutely I mean there are you know 942 00:38:06,170 --> 00:38:03,960 crazy vast distances in space that kind 943 00:38:08,630 --> 00:38:06,180 of bend the human mind but I'm glad you 944 00:38:11,690 --> 00:38:08,640 mentioned book our next question is is 945 00:38:13,849 --> 00:38:11,700 what stories have inspired you to want 946 00:38:15,770 --> 00:38:13,859 to pursue your career and learn more 947 00:38:17,930 --> 00:38:15,780 about life in the universe 948 00:38:20,390 --> 00:38:17,940 well I got to tell you uh it goes back 949 00:38:22,250 --> 00:38:20,400 to fourth grade uh it's I won't tell you 950 00:38:23,990 --> 00:38:22,260 the whole story because it's got more 951 00:38:25,609 --> 00:38:24,000 complicated but fundamentally I was 952 00:38:28,670 --> 00:38:25,619 struggling in fourth grade I didn't like 953 00:38:31,250 --> 00:38:28,680 to read I was bored I was a class clown 954 00:38:33,650 --> 00:38:31,260 in the back you know causing trouble and 955 00:38:35,870 --> 00:38:33,660 we had to buy a book from a book club so 956 00:38:37,910 --> 00:38:35,880 I brought in my 50 cents plopped it down 957 00:38:40,730 --> 00:38:37,920 and I was looking around you know you 958 00:38:43,010 --> 00:38:40,740 can imagine a little kid oh this is so 959 00:38:46,069 --> 00:38:43,020 boring oh and then I came across a title 960 00:38:49,130 --> 00:38:46,079 that said Mission to Mars 961 00:38:50,990 --> 00:38:49,140 and I said huh that sounded like fun so 962 00:38:53,930 --> 00:38:51,000 I bought this book it was about a family 963 00:38:56,089 --> 00:38:53,940 going to Mars and they meet martians the 964 00:38:57,890 --> 00:38:56,099 Martians helped them to survive because 965 00:39:01,609 --> 00:38:57,900 their life support system wasn't working 966 00:39:05,089 --> 00:39:01,619 right and so it's really been fun now to 967 00:39:07,970 --> 00:39:05,099 work and it closes the circle I had a 968 00:39:11,210 --> 00:39:07,980 new spark in fourth grade to think about 969 00:39:12,530 --> 00:39:11,220 outer space and mooc and flying to other 970 00:39:15,010 --> 00:39:12,540 planets 971 00:39:17,270 --> 00:39:15,020 and that came from that one single book 972 00:39:18,829 --> 00:39:17,280 uh and now I'm working on Mars 973 00:39:20,150 --> 00:39:18,839 astrobiology I think that's a really 974 00:39:21,890 --> 00:39:20,160 cool Circle 975 00:39:23,510 --> 00:39:21,900 that is really cool you know you're 976 00:39:24,770 --> 00:39:23,520 speaking to that young kid in me he used 977 00:39:26,569 --> 00:39:24,780 to go to the Scholastic Book Fair and 978 00:39:28,430 --> 00:39:26,579 buy a bunch of books for you know a 979 00:39:30,170 --> 00:39:28,440 nickel or a dime each and then you know 980 00:39:31,609 --> 00:39:30,180 get nerding out over the science and 981 00:39:33,770 --> 00:39:31,619 then then the culture and the history 982 00:39:35,569 --> 00:39:33,780 and all these things exactly um you know 983 00:39:37,130 --> 00:39:35,579 and now in your career it's been quite a 984 00:39:39,770 --> 00:39:37,140 while since fourth grade you've done so 985 00:39:42,470 --> 00:39:39,780 many cool things in your career 986 00:39:44,690 --> 00:39:42,480 if you could though go back to the very 987 00:39:47,329 --> 00:39:44,700 beginning of your career and give 988 00:39:48,950 --> 00:39:47,339 yourself some advice what advice would 989 00:39:51,170 --> 00:39:48,960 you give yourself 990 00:39:54,290 --> 00:39:51,180 wow uh 991 00:39:57,589 --> 00:39:54,300 get done with your graduate work as fast 992 00:40:00,190 --> 00:39:57,599 as possible but doing excellent work 993 00:40:03,410 --> 00:40:00,200 don't dwell on stuff 994 00:40:04,849 --> 00:40:03,420 and get out into the the academic 995 00:40:07,670 --> 00:40:04,859 environment if that's where you want to 996 00:40:12,349 --> 00:40:07,680 go research in Academia or at Nasa 997 00:40:14,750 --> 00:40:12,359 centers or or other National Labs get 998 00:40:17,690 --> 00:40:14,760 your graduate work done quick get out 999 00:40:19,310 --> 00:40:17,700 and start making uh making a name for 1000 00:40:22,790 --> 00:40:19,320 yourself and doing some fun work and the 1001 00:40:25,010 --> 00:40:22,800 second advice is pick really cool field 1002 00:40:28,430 --> 00:40:25,020 work field sites 1003 00:40:30,770 --> 00:40:28,440 so uh doing that Dart that dust 1004 00:40:34,370 --> 00:40:30,780 collection system I was able to fly in a 1005 00:40:35,990 --> 00:40:34,380 f-104 star fighter jet something I've 1006 00:40:39,950 --> 00:40:36,000 wanted to do my whole life since I was 1007 00:40:42,589 --> 00:40:39,960 just a little kid so uh you know just 1008 00:40:44,750 --> 00:40:42,599 have fun and but get to it 1009 00:40:46,069 --> 00:40:44,760 love it so much well let's talk about 1010 00:40:47,210 --> 00:40:46,079 the things that excite you then you know 1011 00:40:49,970 --> 00:40:47,220 when you were a kid you were excited by 1012 00:40:51,530 --> 00:40:49,980 this this f-104 Starfighter 1013 00:40:53,450 --> 00:40:51,540 um but right now you know not just in 1014 00:40:56,030 --> 00:40:53,460 your career and the research you've done 1015 00:40:58,790 --> 00:40:56,040 but just in general what excites you 1016 00:41:02,329 --> 00:40:58,800 about the future 1017 00:41:05,510 --> 00:41:02,339 well I am just totally Blown Away to be 1018 00:41:06,890 --> 00:41:05,520 basically just amazed on the natural 1019 00:41:09,890 --> 00:41:06,900 world 1020 00:41:11,930 --> 00:41:09,900 uh the the big bang happened 3.8 billion 1021 00:41:15,050 --> 00:41:11,940 years ago we started with primarily 1022 00:41:18,109 --> 00:41:15,060 hydrogen and now we have every 1023 00:41:20,990 --> 00:41:18,119 everything that we have uh and there is 1024 00:41:23,390 --> 00:41:21,000 a very logical rational process from 1025 00:41:25,670 --> 00:41:23,400 going from hydrogen gas in the early 1026 00:41:28,010 --> 00:41:25,680 Universe all the way up to complex 1027 00:41:32,750 --> 00:41:28,020 medicine animals like humans and 1028 00:41:34,910 --> 00:41:32,760 giraffes and uh penguins and that that 1029 00:41:38,089 --> 00:41:34,920 whole wide range on how soil chemistry 1030 00:41:39,829 --> 00:41:38,099 played into it evolution of stars Jed 1031 00:41:43,849 --> 00:41:39,839 there's different populations of stars 1032 00:41:46,790 --> 00:41:43,859 metal Rich Stars metal poor Stars uh all 1033 00:41:49,370 --> 00:41:46,800 of that comes together and there's just 1034 00:41:52,150 --> 00:41:49,380 this amazing Universe out there and I 1035 00:41:55,190 --> 00:41:52,160 I'm Blown Away by by some of the 1036 00:41:57,589 --> 00:41:55,200 documentaries that are on TV and in in 1037 00:42:00,589 --> 00:41:57,599 you know broadcast YouTubes and things 1038 00:42:02,450 --> 00:42:00,599 like that it's just wonderful 1039 00:42:04,130 --> 00:42:02,460 yeah definitely and I I was you know I 1040 00:42:05,390 --> 00:42:04,140 was a young kid I would watch like the 1041 00:42:06,890 --> 00:42:05,400 Discovery Channel and The Learning 1042 00:42:08,930 --> 00:42:06,900 Channel back when they had actual 1043 00:42:10,849 --> 00:42:08,940 science content and I would get so like 1044 00:42:11,990 --> 00:42:10,859 just amazed by these documentaries and 1045 00:42:13,970 --> 00:42:12,000 things I remember one from the 90s 1046 00:42:15,650 --> 00:42:13,980 called solar Empire that just like it 1047 00:42:18,470 --> 00:42:15,660 blew my mind thinking about what we were 1048 00:42:20,510 --> 00:42:18,480 learning about science and that brings 1049 00:42:22,970 --> 00:42:20,520 me to my last question in this faster 1050 00:42:25,670 --> 00:42:22,980 than light segment what is a science 1051 00:42:27,710 --> 00:42:25,680 fact that maybe you still find 1052 00:42:29,329 --> 00:42:27,720 unbelievable or that still blows your 1053 00:42:31,490 --> 00:42:29,339 mind 1054 00:42:34,150 --> 00:42:31,500 that we are here 1055 00:42:36,950 --> 00:42:34,160 this can be stated that simply 1056 00:42:40,609 --> 00:42:36,960 uh the big Banks we know when the Big 1057 00:42:43,310 --> 00:42:40,619 Bang started 3.88 billion years ago 1058 00:42:44,810 --> 00:42:43,320 but what's the trigger where'd that 1059 00:42:47,089 --> 00:42:44,820 energy come from 1060 00:42:49,190 --> 00:42:47,099 uh and the fact that again we went from 1061 00:42:51,470 --> 00:42:49,200 hydrogen gas 1062 00:42:52,990 --> 00:42:51,480 through seller Evolution planetary 1063 00:42:56,510 --> 00:42:53,000 Evolution 1064 00:42:58,730 --> 00:42:56,520 biological evolution and here we are a 1065 00:43:00,770 --> 00:42:58,740 species capable of thinking about all of 1066 00:43:03,170 --> 00:43:00,780 that that that's what really sort of 1067 00:43:05,450 --> 00:43:03,180 blows my mind that's beautiful yeah you 1068 00:43:06,829 --> 00:43:05,460 know it kind of blows my mind too 1069 00:43:09,950 --> 00:43:06,839 um just the fact that we get to exist 1070 00:43:11,450 --> 00:43:09,960 for even a short window each of us uh in 1071 00:43:13,430 --> 00:43:11,460 this world this universe you know this 1072 00:43:16,309 --> 00:43:13,440 Cosmos and some of us get to explore it 1073 00:43:17,990 --> 00:43:16,319 together and learn more about our place 1074 00:43:19,010 --> 00:43:18,000 um I really appreciate that so much from 1075 00:43:20,329 --> 00:43:19,020 you 1076 00:43:22,309 --> 00:43:20,339 um I'm going to open it now for our 1077 00:43:24,050 --> 00:43:22,319 audience q a I know we have a bunch of 1078 00:43:26,210 --> 00:43:24,060 questions coming in from YouTube in the 1079 00:43:28,069 --> 00:43:26,220 live chat uh and so let's just get 1080 00:43:29,210 --> 00:43:28,079 started and see what everyone wants to 1081 00:43:31,609 --> 00:43:29,220 ask 1082 00:43:34,250 --> 00:43:31,619 um first starting off uh user at 1083 00:43:36,109 --> 00:43:34,260 rendering reality on Twitter has said 1084 00:43:38,870 --> 00:43:36,119 friendly bacteria like strains of 1085 00:43:41,750 --> 00:43:38,880 bacillus subtilis used for watering or 1086 00:43:44,150 --> 00:43:41,760 foliar feeding make organic farming a 1087 00:43:46,490 --> 00:43:44,160 near closed-loop system is there any 1088 00:43:48,470 --> 00:43:46,500 research using uh good bacteria or 1089 00:43:50,329 --> 00:43:48,480 bacteria from Earth on making an organic 1090 00:43:51,470 --> 00:43:50,339 closed loose loop system for Martian 1091 00:43:54,410 --> 00:43:51,480 farming 1092 00:43:57,950 --> 00:43:54,420 there is actually research in that topic 1093 00:44:00,770 --> 00:43:57,960 uh both in Academia as well as in NASA 1094 00:44:05,829 --> 00:44:00,780 uh these these growth promoting 1095 00:44:07,630 --> 00:44:05,839 beneficial bacteria fungi prokarya 1096 00:44:09,829 --> 00:44:07,640 amoeba 1097 00:44:12,370 --> 00:44:09,839 algae a whole diversity of 1098 00:44:15,890 --> 00:44:12,380 microorganisms are going to be present 1099 00:44:18,829 --> 00:44:15,900 in any lunar biogenerative life support 1100 00:44:21,950 --> 00:44:18,839 system or Mrs bioregenerative life 1101 00:44:23,510 --> 00:44:21,960 support system and we have we have got 1102 00:44:25,910 --> 00:44:23,520 to understand how those beneficial 1103 00:44:27,829 --> 00:44:25,920 organisms are going to interplay with 1104 00:44:30,170 --> 00:44:27,839 the plants and with the humans in these 1105 00:44:32,450 --> 00:44:30,180 closed environments and so because 1106 00:44:36,170 --> 00:44:32,460 humans are will be in so much more 1107 00:44:39,530 --> 00:44:36,180 intimate contact with the life support 1108 00:44:41,510 --> 00:44:39,540 systems and on mars or on the moon uh we 1109 00:44:43,970 --> 00:44:41,520 have there's more to understand on how 1110 00:44:46,550 --> 00:44:43,980 beneficial organisms interact with our 1111 00:44:48,650 --> 00:44:46,560 own microbiomes so there is research 1112 00:44:52,069 --> 00:44:48,660 underway and it's a really exciting area 1113 00:44:54,589 --> 00:44:52,079 of future work for the space program 1114 00:44:58,250 --> 00:44:54,599 wonderful our next question comes from 1115 00:45:00,349 --> 00:44:58,260 Roshan bukhari on YouTube Roshan has 1116 00:45:02,270 --> 00:45:00,359 basically asked what role do you think 1117 00:45:04,309 --> 00:45:02,280 AI will play when it comes to Future 1118 00:45:05,510 --> 00:45:04,319 robotic explorers looking for Life on 1119 00:45:08,270 --> 00:45:05,520 Mars 1120 00:45:10,930 --> 00:45:08,280 oh AI well I'm worried about AI because 1121 00:45:15,770 --> 00:45:10,940 I saw this documentary called Terminator 1122 00:45:18,829 --> 00:45:15,780 and just kidding just kidding uh no AI 1123 00:45:22,670 --> 00:45:18,839 is a very important uh scientific area 1124 00:45:24,650 --> 00:45:22,680 of study and it's so 1125 00:45:27,470 --> 00:45:24,660 um I don't know how to predict how it's 1126 00:45:29,390 --> 00:45:27,480 going to really play out uh so I I 1127 00:45:31,790 --> 00:45:29,400 almost have to say I had no comment on 1128 00:45:35,030 --> 00:45:31,800 this simply because it could be so 1129 00:45:37,730 --> 00:45:35,040 amazing and then yet maybe it doesn't 1130 00:45:40,849 --> 00:45:37,740 really reach the full potential that we 1131 00:45:43,010 --> 00:45:40,859 think it might be able to reach so uh I 1132 00:45:46,910 --> 00:45:43,020 think Advanced Computing is absolutely 1133 00:45:49,089 --> 00:45:46,920 key to successful missions to Mars and 1134 00:45:51,770 --> 00:45:49,099 to the surface of of the Moon 1135 00:45:55,370 --> 00:45:51,780 uh and every time I struggle to get my 1136 00:45:58,010 --> 00:45:55,380 little iPhone to work or or on my TV 1137 00:45:59,990 --> 00:45:58,020 programmed I think golly uh we're gonna 1138 00:46:02,210 --> 00:46:00,000 have trouble going to Mars but AI is 1139 00:46:03,530 --> 00:46:02,220 going to help us solve that 1140 00:46:05,270 --> 00:46:03,540 um that's about all I can say really 1141 00:46:07,490 --> 00:46:05,280 yeah absolutely there's a lot of 1142 00:46:09,470 --> 00:46:07,500 Advanced Technologies not just around 1143 00:46:11,390 --> 00:46:09,480 the corner or coming up tomorrow they're 1144 00:46:13,430 --> 00:46:11,400 here now you know we are using a lot of 1145 00:46:15,530 --> 00:46:13,440 Advanced Technologies and so our next 1146 00:46:16,670 --> 00:46:15,540 question from user tessellated on 1147 00:46:18,650 --> 00:46:16,680 YouTube 1148 00:46:21,050 --> 00:46:18,660 um is about not an advanced technology 1149 00:46:23,030 --> 00:46:21,060 necessarily but one about using tissue 1150 00:46:25,609 --> 00:46:23,040 culture and whether we can apply tissue 1151 00:46:27,650 --> 00:46:25,619 culture to astrobotomy I think for 1152 00:46:29,329 --> 00:46:27,660 myself first I will say you know I've 1153 00:46:30,589 --> 00:46:29,339 talked to people out there who are you 1154 00:46:32,630 --> 00:46:30,599 know in the process of developing 1155 00:46:33,770 --> 00:46:32,640 companies to do things like lab grown 1156 00:46:37,609 --> 00:46:33,780 meat 1157 00:46:39,770 --> 00:46:37,619 making a sushi grade tuna in the 1158 00:46:41,809 --> 00:46:39,780 laboratory or making beef hamburgers in 1159 00:46:43,430 --> 00:46:41,819 the laboratory I do think eventually 1160 00:46:45,309 --> 00:46:43,440 we're going to see people who are 1161 00:46:47,510 --> 00:46:45,319 learning now how to do tissue culture 1162 00:46:49,609 --> 00:46:47,520 we'll be going out with their friends 1163 00:46:51,849 --> 00:46:49,619 who are organic chemists and things like 1164 00:46:54,349 --> 00:46:51,859 this and learning how to develop 1165 00:46:56,030 --> 00:46:54,359 lab-grown Meats for the space station 1166 00:46:57,770 --> 00:46:56,040 and Mars 1167 00:47:00,849 --> 00:46:57,780 um but Dr sugar what do you think of 1168 00:47:03,290 --> 00:47:00,859 using tissue culture in astrobotomy 1169 00:47:04,730 --> 00:47:03,300 well there are many different types of 1170 00:47:06,530 --> 00:47:04,740 tissue culture which you just mentioned 1171 00:47:09,770 --> 00:47:06,540 there's tissue cultures have produced 1172 00:47:11,870 --> 00:47:09,780 pathogen free plantlets that would be 1173 00:47:13,910 --> 00:47:11,880 then propagated into your biogenerative 1174 00:47:16,490 --> 00:47:13,920 life support system I think that's a 1175 00:47:21,010 --> 00:47:16,500 done deal that will be used uh there's 1176 00:47:24,309 --> 00:47:21,020 tissue culture to create uh perhaps 1177 00:47:26,930 --> 00:47:24,319 genetically modified crops that have 1178 00:47:28,730 --> 00:47:26,940 vitamins added in that might not 1179 00:47:31,069 --> 00:47:28,740 normally be there one of the big 1180 00:47:33,290 --> 00:47:31,079 concerns with humans going to Mars is a 1181 00:47:36,530 --> 00:47:33,300 long period of time are we going to be 1182 00:47:38,750 --> 00:47:36,540 able to preserve vitamins you know in 1183 00:47:40,970 --> 00:47:38,760 the normal little pills they begin to 1184 00:47:42,770 --> 00:47:40,980 degrade over a release and reasonable 1185 00:47:45,410 --> 00:47:42,780 short periods of time so we might need 1186 00:47:47,450 --> 00:47:45,420 to grow plants that have vitamin C in 1187 00:47:49,849 --> 00:47:47,460 them that are genetically engineered for 1188 00:47:53,450 --> 00:47:49,859 vitamin C and tissue culture has a role 1189 00:47:56,930 --> 00:47:53,460 to play in that uh having the astronauts 1190 00:47:58,930 --> 00:47:56,940 eat artificial fish and artificial Meats 1191 00:48:02,030 --> 00:47:58,940 that's very very 1192 00:48:05,750 --> 00:48:02,040 scenario although well I'd really hate 1193 00:48:08,569 --> 00:48:05,760 not having a nice rib eye bone-in rib 1194 00:48:11,150 --> 00:48:08,579 eye over cooked over the Barbie so but 1195 00:48:13,730 --> 00:48:11,160 but the technology will be a part of 1196 00:48:15,530 --> 00:48:13,740 these space exploration efforts 1197 00:48:17,089 --> 00:48:15,540 absolutely 1198 00:48:19,609 --> 00:48:17,099 um our next question comes from user 1199 00:48:21,109 --> 00:48:19,619 Haley Monaco on YouTube and it's an 1200 00:48:23,990 --> 00:48:21,119 interesting one um how could we 1201 00:48:26,150 --> 00:48:24,000 differentiate between geological methane 1202 00:48:28,790 --> 00:48:26,160 and biologically produced methane on 1203 00:48:31,790 --> 00:48:28,800 Mars is there a way for us given current 1204 00:48:35,030 --> 00:48:31,800 technology to differentiate 1205 00:48:37,010 --> 00:48:35,040 yeah we can do that now on Earth uh and 1206 00:48:40,430 --> 00:48:37,020 it's really due to the isotopic 1207 00:48:43,849 --> 00:48:40,440 fractionation it's called Uh carbon 12 1208 00:48:46,430 --> 00:48:43,859 is the primary carbon atom that makes 1209 00:48:48,829 --> 00:48:46,440 all of us uh there's a small amount of 1210 00:48:51,470 --> 00:48:48,839 carbon 13 which has one extra Neutron 1211 00:48:52,510 --> 00:48:51,480 and then even a smaller amount of carbon 1212 00:48:55,150 --> 00:48:52,520 14 1213 00:48:59,089 --> 00:48:55,160 and biological 1214 00:49:03,130 --> 00:48:59,099 methane has a different carbon 12 to 13 1215 00:49:06,250 --> 00:49:03,140 to 14 ratio then 1216 00:49:09,890 --> 00:49:06,260 abiogenic methane generally speaking 1217 00:49:12,770 --> 00:49:09,900 abiogenic methane will not be enriched 1218 00:49:15,890 --> 00:49:12,780 in carbon 13 or carbon 14 it'll be sort 1219 00:49:19,790 --> 00:49:15,900 of a straight ratio of you know I think 1220 00:49:22,430 --> 00:49:19,800 it's I think it's 98 carbon 12. 1.9 1221 00:49:25,250 --> 00:49:22,440 percent carbon 13 and then 0.1 percent 1222 00:49:27,829 --> 00:49:25,260 carbon 14. but biology has the ability 1223 00:49:29,930 --> 00:49:27,839 to enrich some of those those Isotopes 1224 00:49:32,089 --> 00:49:29,940 and so that's one way to tell the 1225 00:49:34,250 --> 00:49:32,099 difference between biogenic methane and 1226 00:49:36,349 --> 00:49:34,260 abiogenic methane 1227 00:49:38,270 --> 00:49:36,359 yeah absolutely then that's important 1228 00:49:40,309 --> 00:49:38,280 it's also important to know the source 1229 00:49:42,170 --> 00:49:40,319 of the the carbon isotope reservoirs 1230 00:49:43,970 --> 00:49:42,180 that we might have on Mars and so that 1231 00:49:45,410 --> 00:49:43,980 will require a lot of work too figuring 1232 00:49:48,530 --> 00:49:45,420 out you know what are we starting with 1233 00:49:49,849 --> 00:49:48,540 for isotopic uh abundances on Mars when 1234 00:49:51,349 --> 00:49:49,859 we have a sample the methane's coming 1235 00:49:54,230 --> 00:49:51,359 from 1236 00:49:57,230 --> 00:49:54,240 um now we do have a user Mark Lumsden on 1237 00:49:59,270 --> 00:49:57,240 Twitter has asked a question if we have 1238 00:50:01,430 --> 00:49:59,280 any evidence of Life possibly being 1239 00:50:02,990 --> 00:50:01,440 based on different chemistry 1240 00:50:05,089 --> 00:50:03,000 um people often often ask you know like 1241 00:50:07,190 --> 00:50:05,099 carbon based life versus silicon-based 1242 00:50:09,410 --> 00:50:07,200 life could there be other solvents out 1243 00:50:11,329 --> 00:50:09,420 there besides water I will personally 1244 00:50:13,430 --> 00:50:11,339 say an answer to Mark 1245 00:50:14,870 --> 00:50:13,440 um silicon-based life is a possibility 1246 00:50:17,630 --> 00:50:14,880 it might be that we actually create 1247 00:50:19,370 --> 00:50:17,640 maybe post biological life on Earth will 1248 00:50:20,690 --> 00:50:19,380 be silicon based because we've made it 1249 00:50:22,010 --> 00:50:20,700 that way 1250 00:50:24,410 --> 00:50:22,020 um it is worth looking into the 1251 00:50:25,730 --> 00:50:24,420 chemistry though silicon silicon bonds 1252 00:50:28,430 --> 00:50:25,740 are not nearly as strong as 1253 00:50:31,670 --> 00:50:28,440 carbon-carbon bonds and they're also not 1254 00:50:32,450 --> 00:50:31,680 super stable in water like carbon is and 1255 00:50:35,569 --> 00:50:32,460 so there might be a different 1256 00:50:36,890 --> 00:50:35,579 environment a product cryosolvins for 1257 00:50:38,750 --> 00:50:36,900 instance are a place where silicon 1258 00:50:40,790 --> 00:50:38,760 silicon bonds are much stronger and can 1259 00:50:42,530 --> 00:50:40,800 survive a lot better and so it's worth 1260 00:50:44,349 --> 00:50:42,540 looking into the chemistry if you're 1261 00:50:48,410 --> 00:50:44,359 really interested in this question mark 1262 00:50:50,510 --> 00:50:48,420 however Andrew for you our next user THM 1263 00:50:53,089 --> 00:50:50,520 on YouTube has said that they're 1264 00:50:55,670 --> 00:50:53,099 interested in inter researching microbes 1265 00:50:57,230 --> 00:50:55,680 living in lava tubes do you have any 1266 00:50:59,390 --> 00:50:57,240 recommendations for them of what they 1267 00:51:01,190 --> 00:50:59,400 might want to look into when it comes to 1268 00:51:04,549 --> 00:51:01,200 understanding how microbes would thrive 1269 00:51:06,829 --> 00:51:04,559 in a unique environment like that 1270 00:51:08,690 --> 00:51:06,839 wow that that's a very good topic I 1271 00:51:10,309 --> 00:51:08,700 sometimes think back on a discipline 1272 00:51:11,809 --> 00:51:10,319 that I wish I would have gone into from 1273 00:51:14,150 --> 00:51:11,819 a career standpoint and Cave 1274 00:51:16,849 --> 00:51:14,160 microbiology is one of that one of those 1275 00:51:19,910 --> 00:51:16,859 topics I've been into some caves in the 1276 00:51:22,190 --> 00:51:19,920 southwest of the United States that uh 1277 00:51:24,049 --> 00:51:22,200 on the outside of the cave there's it's 1278 00:51:27,530 --> 00:51:24,059 dry hot 1279 00:51:30,109 --> 00:51:27,540 of desert terrain desert ecosystems you 1280 00:51:32,510 --> 00:51:30,119 drop down into the cave and it there's 1281 00:51:35,150 --> 00:51:32,520 literally fog in the cave just just a 1282 00:51:37,190 --> 00:51:35,160 few tens of meters from The Cave opening 1283 00:51:39,530 --> 00:51:37,200 there's literally fog forming 1284 00:51:42,130 --> 00:51:39,540 condensation on the walls with an 1285 00:51:45,069 --> 00:51:42,140 incredible diversity of microbial 1286 00:51:48,349 --> 00:51:45,079 species on the walls and on the ceilings 1287 00:51:49,970 --> 00:51:48,359 uh when I first saw that you know 10 1288 00:51:52,250 --> 00:51:49,980 years ago on a field trip at a 1289 00:51:56,150 --> 00:51:52,260 conference it was like wow that's so 1290 00:51:58,849 --> 00:51:56,160 exciting uh how to get into it the best 1291 00:52:01,790 --> 00:51:58,859 thing to do on any discipline of how to 1292 00:52:04,970 --> 00:52:01,800 get into it is look into the literature 1293 00:52:07,010 --> 00:52:04,980 on who's publishing in the last few 1294 00:52:10,010 --> 00:52:07,020 years on your topic 1295 00:52:13,190 --> 00:52:10,020 so if you find papers of a scientist 1296 00:52:16,250 --> 00:52:13,200 that's published you know on the 1297 00:52:17,870 --> 00:52:16,260 Southwest lava tube caves in a variety 1298 00:52:19,549 --> 00:52:17,880 of locations 1299 00:52:23,750 --> 00:52:19,559 contact them 1300 00:52:25,970 --> 00:52:23,760 uh if they're if their literature is is 1301 00:52:28,609 --> 00:52:25,980 not in your topical area but it's in 1302 00:52:30,770 --> 00:52:28,619 cave microbiology and but what they're 1303 00:52:32,630 --> 00:52:30,780 doing is not that interesting contact 1304 00:52:35,270 --> 00:52:32,640 them and say this is what I'm interested 1305 00:52:38,089 --> 00:52:35,280 in doing who would you might recommend 1306 00:52:40,309 --> 00:52:38,099 that would be a different Professor uh 1307 00:52:43,670 --> 00:52:40,319 and so the best thing is just get into 1308 00:52:46,970 --> 00:52:43,680 it get find out what you're interested 1309 00:52:48,829 --> 00:52:46,980 in and write to the people and ask for a 1310 00:52:51,589 --> 00:52:48,839 little bit of advice they mostly will 1311 00:52:54,049 --> 00:52:51,599 will respond to you that's fantastic and 1312 00:52:55,910 --> 00:52:54,059 great advice one way to find really good 1313 00:52:57,290 --> 00:52:55,920 mentors and advisors out there is just 1314 00:52:58,309 --> 00:52:57,300 to reach out to people who are doing the 1315 00:52:59,390 --> 00:52:58,319 work 1316 00:53:01,130 --> 00:52:59,400 um but it's also a great way to make 1317 00:53:03,349 --> 00:53:01,140 those connections to find people who can 1318 00:53:04,430 --> 00:53:03,359 help you uh find the research and so I 1319 00:53:06,410 --> 00:53:04,440 really appreciate that as well it's 1320 00:53:07,910 --> 00:53:06,420 always advice that I love to hear given 1321 00:53:10,309 --> 00:53:07,920 to people 1322 00:53:12,770 --> 00:53:10,319 um our next question comes from astativa 1323 00:53:14,510 --> 00:53:12,780 arai on YouTube 1324 00:53:15,650 --> 00:53:14,520 um and I'm gonna change their question a 1325 00:53:17,750 --> 00:53:15,660 little bit they want they want to know 1326 00:53:20,089 --> 00:53:17,760 how important is the Criterion of 1327 00:53:24,109 --> 00:53:20,099 salinity in the Martian soil when it 1328 00:53:25,609 --> 00:53:24,119 comes to living things including growing 1329 00:53:27,230 --> 00:53:25,619 um and so for the Martian and we call it 1330 00:53:31,549 --> 00:53:27,240 regolith 1331 00:53:34,190 --> 00:53:31,559 know salinity but how important are the 1332 00:53:35,870 --> 00:53:34,200 actual salts themselves for instance I 1333 00:53:37,670 --> 00:53:35,880 know an astrobotonist who has pointed 1334 00:53:39,470 --> 00:53:37,680 out that you know in the Martian that 1335 00:53:40,549 --> 00:53:39,480 Mark Watney eating those potatoes would 1336 00:53:42,710 --> 00:53:40,559 have been in trouble because he didn't 1337 00:53:45,410 --> 00:53:42,720 clean the perchlorate salts out of the 1338 00:53:46,790 --> 00:53:45,420 Martian regalith first so I wonder uh Dr 1339 00:53:49,069 --> 00:53:46,800 sugar if you can speak to the Martian 1340 00:53:50,809 --> 00:53:49,079 regolith it's chemistry and maybe how 1341 00:53:53,809 --> 00:53:50,819 important some of these salts are 1342 00:53:55,970 --> 00:53:53,819 right uh boy that that could be a whole 1343 00:53:58,309 --> 00:53:55,980 topic an hour long topic just on the 1344 00:54:01,609 --> 00:53:58,319 geochemistry of the Martian regolith uh 1345 00:54:04,370 --> 00:54:01,619 the main thing is that the salts uh on 1346 00:54:07,430 --> 00:54:04,380 Mars the regolith is composed of ground 1347 00:54:10,670 --> 00:54:07,440 up basaltic material that's been 1348 00:54:14,930 --> 00:54:10,680 weathered or acted upon over geological 1349 00:54:18,109 --> 00:54:14,940 times with water water vapor 1350 00:54:21,290 --> 00:54:18,119 um and other uh energy sources like UV 1351 00:54:23,230 --> 00:54:21,300 irradiation with water vapor with mixing 1352 00:54:27,349 --> 00:54:23,240 of minerals so there is actual 1353 00:54:29,089 --> 00:54:27,359 weathering of the soils much more than 1354 00:54:31,250 --> 00:54:29,099 on the moon and here's a great example I 1355 00:54:35,089 --> 00:54:31,260 got to use my little my little show and 1356 00:54:38,569 --> 00:54:35,099 tell here in this hand is a gray lunar 1357 00:54:40,670 --> 00:54:38,579 simulant that I use in my lab this is a 1358 00:54:44,089 --> 00:54:40,680 primary mineral it's just like ground up 1359 00:54:46,609 --> 00:54:44,099 Basalt which is like lava okay this is 1360 00:54:49,250 --> 00:54:46,619 the Martian stimulant and it is 1361 00:54:50,750 --> 00:54:49,260 orange-like now the chemistries of these 1362 00:54:53,690 --> 00:54:50,760 two are very similar they're basic 1363 00:54:56,089 --> 00:54:53,700 chemistry this is basaltic this is 1364 00:54:58,030 --> 00:54:56,099 basaltic but what's happened here with 1365 00:55:02,089 --> 00:54:58,040 the Martian stimulant 1366 00:55:05,450 --> 00:55:02,099 is that water and oxygen have acted upon 1367 00:55:08,150 --> 00:55:05,460 this material over geological time and 1368 00:55:12,349 --> 00:55:08,160 have altered it such that it's more 1369 00:55:14,210 --> 00:55:12,359 complex in its geochemistry it is has a 1370 00:55:16,370 --> 00:55:14,220 lot more salts in it the salts are 1371 00:55:18,589 --> 00:55:16,380 forming and dissolving and forming and 1372 00:55:20,569 --> 00:55:18,599 changing because of this liquid water 1373 00:55:23,390 --> 00:55:20,579 environment that's hap that's been on 1374 00:55:26,270 --> 00:55:23,400 Mars in the past so these two little 1375 00:55:27,950 --> 00:55:26,280 soil samples are very different but yet 1376 00:55:32,569 --> 00:55:27,960 they started out at the same which is 1377 00:55:35,270 --> 00:55:32,579 basalt and so on Mars there is a problem 1378 00:55:38,650 --> 00:55:35,280 with some of these soils might have 1379 00:55:41,809 --> 00:55:38,660 salts that are too high a concentration 1380 00:55:44,510 --> 00:55:41,819 to use those soils immediately for 1381 00:55:47,150 --> 00:55:44,520 growing plants in so we might have to 1382 00:55:49,430 --> 00:55:47,160 figure out methods of leeching out the 1383 00:55:51,410 --> 00:55:49,440 salts or chemically extracting the salts 1384 00:55:53,690 --> 00:55:51,420 from the soils before they can be used 1385 00:55:55,849 --> 00:55:53,700 to grow plants on so that's one thing 1386 00:55:57,230 --> 00:55:55,859 the other thing is that the salts 1387 00:55:59,750 --> 00:55:57,240 themselves the little tiny micro 1388 00:56:01,430 --> 00:55:59,760 crystals of salts can land on a 1389 00:56:04,430 --> 00:56:01,440 spacecraft surface and if they come into 1390 00:56:06,770 --> 00:56:04,440 contact with a bacterial cell that 1391 00:56:08,150 --> 00:56:06,780 little super Crystal of salt can 1392 00:56:10,549 --> 00:56:08,160 actually kill a lot of different 1393 00:56:12,950 --> 00:56:10,559 bacterial cells just by contacting you 1394 00:56:16,250 --> 00:56:12,960 don't even need liquid water to have the 1395 00:56:17,990 --> 00:56:16,260 salt dissolve in so salts are one of 1396 00:56:20,630 --> 00:56:18,000 those biocidal factors that are on the 1397 00:56:24,049 --> 00:56:20,640 in the Martian regolith they can kill 1398 00:56:25,910 --> 00:56:24,059 bacteria and spacecraft surfaces and we 1399 00:56:28,190 --> 00:56:25,920 might have to learn how to remove the 1400 00:56:29,870 --> 00:56:28,200 salts before we can use the regulate for 1401 00:56:31,730 --> 00:56:29,880 growth and that's actually a really 1402 00:56:34,010 --> 00:56:31,740 important area of research that needs to 1403 00:56:35,270 --> 00:56:34,020 be done yeah absolutely it's so 1404 00:56:37,490 --> 00:56:35,280 important for us to know how we could 1405 00:56:39,470 --> 00:56:37,500 use that regolith to grow and to use as 1406 00:56:42,230 --> 00:56:39,480 a building material as well to you know 1407 00:56:44,150 --> 00:56:42,240 to reinforce our habitats to things like 1408 00:56:46,190 --> 00:56:44,160 that to build habitats 1409 00:56:47,990 --> 00:56:46,200 um there's so much to learn our next 1410 00:56:52,510 --> 00:56:48,000 question for you comes from Milton 1411 00:56:55,010 --> 00:56:52,520 mendocha Jr at Ms mendocha Jr on Twitter 1412 00:56:57,410 --> 00:56:55,020 uh they want to know that if there is 1413 00:56:59,930 --> 00:56:57,420 life on Mars Martian life not earth life 1414 00:57:02,270 --> 00:56:59,940 but it's not based on things like DNA 1415 00:57:04,010 --> 00:57:02,280 and proteins would there still be a way 1416 00:57:05,569 --> 00:57:04,020 for us to understand 1417 00:57:07,730 --> 00:57:05,579 um biological things like how the 1418 00:57:10,150 --> 00:57:07,740 resisting radiation salinity maybe even 1419 00:57:13,750 --> 00:57:10,160 if they're there 1420 00:57:16,970 --> 00:57:13,760 well uh I think the scientific Community 1421 00:57:18,770 --> 00:57:16,980 believes or uses the definition of life 1422 00:57:21,950 --> 00:57:18,780 that requires 1423 00:57:24,109 --> 00:57:21,960 a series of steps that leads to 1424 00:57:26,690 --> 00:57:24,119 Evolution adaptation 1425 00:57:29,270 --> 00:57:26,700 so life has to be able to take in 1426 00:57:32,510 --> 00:57:29,280 whatever liquid they're using as their 1427 00:57:35,270 --> 00:57:32,520 medium in our case it's water life has 1428 00:57:38,990 --> 00:57:35,280 to take in nutrients life has to have 1429 00:57:41,510 --> 00:57:39,000 the mechanisms within the cell or cells 1430 00:57:44,210 --> 00:57:41,520 to take those raw materials and make 1431 00:57:46,730 --> 00:57:44,220 whatever they need to make in order to 1432 00:57:48,609 --> 00:57:46,740 divide and then keep the process going 1433 00:57:51,670 --> 00:57:48,619 through multiple cycles 1434 00:57:54,890 --> 00:57:51,680 and that process 1435 00:57:56,710 --> 00:57:54,900 requires a road map a genetic road map 1436 00:57:59,390 --> 00:57:56,720 that could be DNA 1437 00:58:02,630 --> 00:57:59,400 uh well in our case it's DNA but it 1438 00:58:04,790 --> 00:58:02,640 could also be DNA on the surface of Mars 1439 00:58:08,089 --> 00:58:04,800 because there's our two planets of 1440 00:58:10,549 --> 00:58:08,099 exchanged Rock debris early in our in 1441 00:58:13,010 --> 00:58:10,559 our history and continue to this day so 1442 00:58:15,589 --> 00:58:13,020 if life was present on an early Earth it 1443 00:58:21,230 --> 00:58:15,599 could have been transported to Mars and 1444 00:58:23,750 --> 00:58:21,240 or vice versa uh so life requires this 1445 00:58:27,290 --> 00:58:23,760 process and at the end of that process 1446 00:58:30,109 --> 00:58:27,300 is evolution so if there is an extant 1447 00:58:32,630 --> 00:58:30,119 microbiota on the surface of Mars or in 1448 00:58:34,730 --> 00:58:32,640 the shallow subsurface it has to have 1449 00:58:37,370 --> 00:58:34,740 those ingredients it has to be able to 1450 00:58:39,770 --> 00:58:37,380 acquire nutrients which means we should 1451 00:58:41,930 --> 00:58:39,780 be able to figure out what it needs as a 1452 00:58:43,730 --> 00:58:41,940 set of nutrients it's going to be 1453 00:58:45,770 --> 00:58:43,740 sensitive to some things and not to 1454 00:58:47,630 --> 00:58:45,780 others and we should be able to figure 1455 00:58:51,950 --> 00:58:47,640 that out 1456 00:58:54,650 --> 00:58:51,960 um the real question is on Earth 1457 00:58:57,289 --> 00:58:54,660 greater than 99 of the microorganisms 1458 00:58:58,789 --> 00:58:57,299 that are in our environment are not a 1459 00:58:59,809 --> 00:58:58,799 we're not able to grow them in the 1460 00:59:02,270 --> 00:58:59,819 laboratory 1461 00:59:04,670 --> 00:59:02,280 uh so that creates quite a lot of 1462 00:59:07,549 --> 00:59:04,680 difficulty in looking at their basic 1463 00:59:09,829 --> 00:59:07,559 metabolism basic methods of evolution 1464 00:59:11,809 --> 00:59:09,839 and adaptation there are methods to 1465 00:59:15,410 --> 00:59:11,819 doing that and I think those same 1466 00:59:18,170 --> 00:59:15,420 methods can be applied to any microbes 1467 00:59:20,870 --> 00:59:18,180 on Mars that are not cultural but the 1468 00:59:24,230 --> 00:59:20,880 last point of this question is that like 1469 00:59:27,470 --> 00:59:24,240 Earth Mars if there's life has a series 1470 00:59:29,690 --> 00:59:27,480 of ecosystems it's not just a single 1471 00:59:32,210 --> 00:59:29,700 bacterium sitting on a rock saying hey I 1472 00:59:34,010 --> 00:59:32,220 hope I get sunlight today there's an 1473 00:59:36,289 --> 00:59:34,020 ecosystem there and there has to be 1474 00:59:38,770 --> 00:59:36,299 energy flow within that ecosystem and it 1475 00:59:41,329 --> 00:59:38,780 has to be chemical flow and 1476 00:59:44,329 --> 00:59:41,339 disequilibrium's uh creating the ability 1477 00:59:46,430 --> 00:59:44,339 to do work in that ecosystem that is 1478 00:59:48,650 --> 00:59:46,440 something that's really complex and it's 1479 00:59:51,829 --> 00:59:48,660 it's like we should be able to see that 1480 00:59:54,349 --> 00:59:51,839 if there's life then there's ecosystems 1481 00:59:57,170 --> 00:59:54,359 and we should be able to detect those I 1482 01:00:01,870 --> 00:59:57,180 believe without without invoking very 1483 01:00:04,490 --> 01:00:01,880 unusual exotic silly based life or 1484 01:00:07,130 --> 01:00:04,500 methane-based life at you know minus 300 1485 01:00:09,170 --> 01:00:07,140 degrees or whatever 1486 01:00:11,450 --> 01:00:09,180 that's awesome uh so I so much 1487 01:00:12,770 --> 01:00:11,460 appreciate your time and joining us here 1488 01:00:14,750 --> 01:00:12,780 um I apologize though it's been great 1489 01:00:16,789 --> 01:00:14,760 then you have so many more questions but 1490 01:00:18,109 --> 01:00:16,799 we are at the very top of the hour 1491 01:00:20,870 --> 01:00:18,119 um if I could just ask for a really 1492 01:00:22,309 --> 01:00:20,880 brief answer to one more question 1493 01:00:24,770 --> 01:00:22,319 um I saw one that I really liked from 1494 01:00:27,589 --> 01:00:24,780 kashish Gupta on YouTube 1495 01:00:29,690 --> 01:00:27,599 um so so kashish has asked Dr sugar if 1496 01:00:31,609 --> 01:00:29,700 you get to send a single microbial 1497 01:00:34,970 --> 01:00:31,619 species on a future Mission to Mars 1498 01:00:37,250 --> 01:00:34,980 which one would you pick 1499 01:00:39,589 --> 01:00:37,260 wow I don't want to send any microbes to 1500 01:00:40,430 --> 01:00:39,599 Mars 1501 01:00:48,530 --> 01:00:40,440 um 1502 01:00:49,789 --> 01:00:48,540 starting the process of terraforming the 1503 01:00:52,970 --> 01:00:49,799 planet 1504 01:00:55,190 --> 01:00:52,980 if I was if if we were if we understood 1505 01:00:58,150 --> 01:00:55,200 the environment on Mars enough and 1506 01:01:01,609 --> 01:00:58,160 humanity is ready to take the step 1507 01:01:04,370 --> 01:01:01,619 towards uh you know terraforming the 1508 01:01:07,069 --> 01:01:04,380 surface of Mars my first thought would 1509 01:01:08,630 --> 01:01:07,079 be to begin sending microbes I could 1510 01:01:11,150 --> 01:01:08,640 take carbon dioxide out of the air 1511 01:01:14,450 --> 01:01:11,160 produce oxygen maybe even utilize the 1512 01:01:15,890 --> 01:01:14,460 nitrogen uh so that that would be I 1513 01:01:18,109 --> 01:01:15,900 think the most intriguing but that's 1514 01:01:19,069 --> 01:01:18,119 tens of years away if not hundreds of 1515 01:01:21,289 --> 01:01:19,079 years away 1516 01:01:22,609 --> 01:01:21,299 absolutely yeah well thank you so much 1517 01:01:23,809 --> 01:01:22,619 for that answer 1518 01:01:25,250 --> 01:01:23,819 um to the rest of our audience I 1519 01:01:26,750 --> 01:01:25,260 apologize we couldn't get to all of your 1520 01:01:27,650 --> 01:01:26,760 questions there were so many good ones 1521 01:01:29,089 --> 01:01:27,660 there 1522 01:01:30,410 --> 01:01:29,099 um if you are interested uh you can 1523 01:01:32,329 --> 01:01:30,420 always ask your questions in the 1524 01:01:34,730 --> 01:01:32,339 comments section once we post the 1525 01:01:36,109 --> 01:01:34,740 recorded version of the YouTube uh video 1526 01:01:37,430 --> 01:01:36,119 as well 1527 01:01:38,930 --> 01:01:37,440 um for those of you out there who watch 1528 01:01:40,609 --> 01:01:38,940 the show and join in and ask us 1529 01:01:42,829 --> 01:01:40,619 questions please share the show with 1530 01:01:44,870 --> 01:01:42,839 your friends uh we love to always point 1531 01:01:46,970 --> 01:01:44,880 out an ambassador of the month for each 1532 01:01:48,650 --> 01:01:46,980 episode who's shared about the show 1533 01:01:50,990 --> 01:01:48,660 who's asked questions of our guests 1534 01:01:53,210 --> 01:01:51,000 who's really gotten engaged uh in this 1535 01:01:54,710 --> 01:01:53,220 whole thing of astrobiology we want to 1536 01:01:57,890 --> 01:01:54,720 give a special shout out this month for 1537 01:01:59,270 --> 01:01:57,900 instance to father karuya fafi is an 1538 01:02:01,490 --> 01:01:59,280 affiliate researcher at Blue Marble 1539 01:02:03,470 --> 01:02:01,500 space Institute of science as well as 1540 01:02:05,690 --> 01:02:03,480 being a researcher at NASA Ames and he 1541 01:02:08,690 --> 01:02:05,700 shared about our show so father we 1542 01:02:10,309 --> 01:02:08,700 really appreciate that uh Dr sugar thank 1543 01:02:12,109 --> 01:02:10,319 you so much for joining us for ask an 1544 01:02:13,549 --> 01:02:12,119 astrobiologist it's been a blast having 1545 01:02:15,530 --> 01:02:13,559 you it's been fun 1546 01:02:17,809 --> 01:02:15,540 this has been fun thanks 1547 01:02:19,490 --> 01:02:17,819 thank you so much I'm glad our audience 1548 01:02:21,109 --> 01:02:19,500 has so many great questions about your 1549 01:02:23,210 --> 01:02:21,119 research they might even reach out with 1550 01:02:25,190 --> 01:02:23,220 more questions so keep an eye out for 1551 01:02:26,870 --> 01:02:25,200 that uh for those of you tuning in and 1552 01:02:29,150 --> 01:02:26,880 joining us I hope you signed up already 1553 01:02:30,950 --> 01:02:29,160 for the NASA astrobiology mailing list 1554 01:02:33,289 --> 01:02:30,960 that way you can find out about new 1555 01:02:34,970 --> 01:02:33,299 episodes of ask an astrobiologist as 1556 01:02:36,530 --> 01:02:34,980 well as all of the other incredible 1557 01:02:39,230 --> 01:02:36,540 things going on through NASA 1558 01:02:41,990 --> 01:02:39,240 astrobiology including opportunities for 1559 01:02:43,789 --> 01:02:42,000 early career researchers missions new 1560 01:02:45,890 --> 01:02:43,799 articles on the website lots of cool 1561 01:02:48,170 --> 01:02:45,900 things going on there so please sign up 1562 01:02:50,150 --> 01:02:48,180 for the official mailing list uh thank 1563 01:02:52,910 --> 01:02:50,160 you Dr sugar for joining us thank you to 1564 01:02:57,470 --> 01:02:52,920 the audience for joining us uh and as 1565 01:03:00,010 --> 01:02:57,480 always everyone remember to stay curious 1566 01:03:14,340 --> 01:03:00,020 foreign 1567 01:03:15,450 --> 01:03:14,350 [Music] 1568 01:03:16,500 --> 01:03:15,460 [Applause] 1569 01:03:18,400 --> 01:03:16,510 [Music] 1570 01:03:27,549 --> 01:03:18,410 [Applause] 1571 01:03:39,970 --> 01:03:30,210 thank you